OriGene

NEK6 Monoclonal Antibody (OTI5D7), TrueMAB™

Product Image Gallery

NEK6 Antibody in Western Blot (WB) — HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NEK6 (RC203609, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-NEK6. Positive lysates LY402327 (100 µg) and LC402327 (20 µg) can be purchased separately from OriGene.

NEK6 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-NEK6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500545)

NEK6 Antibody in Flow Cytometry (Flow) — HEK293T cells transfected with either RC203609 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-NEK6 antibody (TA500545), and then analyzed by flow cytometry.

NEK6 Antibody in Immunocytochemistry (ICC/IF) — Anti-NEK6 mouse monoclonal antibody (TA500545) Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NEK6(RC203609).

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2000

Immunohistochemistry (Paraffin) (IHC (P))

1:50

Flow Cytometry (Flow)

1:100

Immunocytochemistry (ICC/IF)

1:100

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

OTI5D7

Immunogen

Full length human recombinant protein of human NEK6 produced in HEK293T cell.

Conjugate

Unconjugated

Form

lyophilized

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 8% trehalose

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Product Specific Information

Reconstitute with PBS (pH 7.3) and recommend to perform another round of desalting process using Product # 7KMWCO

Target Information

The Aspergillus nidulans 'never in mitosis A' (NIMA) gene encodes a serine/threonine kinase that controls initiation of mitosis. NIMA-related kinases (NEKs) are a group of protein kinases that are homologous to NIMA. Evidence suggests that NEKs perform functions similar to those of NIMA. NEK6 encodes a NEK type protein kinase, which contains a C-terminal NIMA-like catalytic domain.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Never in mitosis A-related kinase 6; NIMA (never in mitosis gene a)-related kinase 6; NIMA-related kinase 6; NimA-related protein kinase 6; Protein kinase SID6-1512; putative serine-threonine protein kinase; RP11-101K10.6; Serine/threonine-protein kinase Nek6; SID

Gene Aliases: NEK6; SID6-1512

UniProt ID: (Human) Q9HC98

Entrez Gene ID: (Human) 10783

Molecular Function: kinase protein kinase transferase