OriGene

NEK6 Monoclonal Antibody (OTI5D7), TrueMAB™

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NEK6 Antibody in Immunocytochemistry (ICC/IF) — Anti-NEK6 mouse monoclonal antibody (TA500545) Immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NEK6(RC203609).

NEK6 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-NEK6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500545)

NEK6 Antibody in Western Blot (WB) — HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NEK6 (RC203609, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-NEK6. Positive lysates LY402327 (100 µg) and LC402327 (20 µg) can be purchased separately from OriGene.

NEK6 Antibody in Flow Cytometry (Flow) — HEK293T cells transfected with either RC203609 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-NEK6 antibody (TA500545), and then analyzed by flow cytometry.

Product Details

CF500545

Applications	Tested Dilution	Publications
Western Blot (WB)	1:2,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:50	-
Immunocytochemistry (ICC/IF)	1:100	-
Flow Cytometry (Flow)	1:100	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Clone	OTI5D7
Immunogen	Full length human recombinant protein of human NEK6 produced in HEK293T cell. View antigen
Conjugate	Unconjugated
Form	lyophilized
Concentration	1 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.3, with 8% trehalose
Contains	no preservative
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles

Product Specific Information

For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)

Target Information

The Aspergillus nidulans 'never in mitosis A' (NIMA) gene encodes a serine/threonine kinase that controls initiation of mitosis. NIMA-related kinases (NEKs) are a group of protein kinases that are homologous to NIMA. Evidence suggests that NEKs perform functions similar to those of NIMA. NEK6 encodes a NEK type protein kinase, which contains a C-terminal NIMA-like catalytic domain.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Never in mitosis A-related kinase 6; NIMA (never in mitosis gene a)-related kinase 6; NIMA-related kinase 6; NimA-related protein kinase 6; Protein kinase SID6-1512; putative serine-threonine protein kinase; RP11-101K10.6; Serine/threonine-protein kinase Nek6; SID

Gene Aliases: NEK6; SID6-1512

UniProt ID: (Human) Q9HC98

Entrez Gene ID: (Human) 10783

Molecular Function: non-receptor serine/threonine protein kinase