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NEK7 Antibody (MA5-38018) in WB
Western blot analysis of NEK7 in extracts of various cell lines. Samples were incubated with NEK7 Monoclonal antibody (Product # MA5-38018) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Product Details
MA5-38018
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
ARC2342
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 203-302 of human NEK7 (Q8TDX7)
Conjugate
Form
Liquid
Concentration
0.54 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 0.05% BSA, 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2897936
Product Specific Information
Positive Samples: HeLa, MCF7, NIH/3T3, C6, Mouse lung, Mouse liver
Immunogen sequence: MSPERIHENG YNFKSDIWSL GCLLYEMAAL QSPFYGDKMN LYSLCKKIEQ CDYPPLPSDH YSEELRQLVN MCINPDPEKR PDVTYVYDVA KRMHACTASS
Target Information
NEK7 (NIMA Related Kinase 7) is a Protein Coding gene. Diseases associated with NEK7 include Muckle-Wells Syndrome and Cinca Syndrome. Among its related pathways are Cell Cycle, Mitotic and Transport of the SLBP independent Mature mRNA. Gene Ontology (GO) annotations related to this gene include transferase activity, transferring phosphorus-containing groups and protein tyrosine kinase activity. An important paralog of this gene is NEK6.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Never in mitosis A-related kinase 7; NIMA (never in mitosis gene a)-related expressed kinase 7; NIMA (never in mitosis gene a)-related kinase 7; NIMA-related expressed kinase 7; NIMA-related kinase 7; NimA-related protein kinase 7; Serine/threonine-protein kinase Nek7
Gene Aliases: 2810460C19Rik; AU020186; NEK7
UniProt ID: (Human) Q8TDX7, (Rat) D3ZBE5, (Mouse) Q9ES74
Entrez Gene ID: (Human) 140609, (Rat) 360850, (Mouse) 59125
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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