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Product Details
PA5-20577
| Applications | Tested Dilution | Publications |
|---|---|---|
Western Blot (WB) |
1-2 µg/mL | - |
Immunohistochemistry (IHC) |
10 µg/mL | - |
| Product Specifications | |
|---|---|
Species Reactivity |
Human, Mouse, Rat |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
A 15 amino acid peptide from near the carboxy terminus of human NENF. |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
1 mg/mL |
Purification |
Antigen affinity chromatography |
Storage buffer |
PBS |
Contains |
0.02% sodium azide |
Storage conditions |
Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C |
RRID |
AB_11153616 |
Product Specific Information
Despite its predicted molecular weight of ~20kDa, NENF is observed at 37kDa in SDS-PAGE. A suggested positive control is human small intestine tissue lysate.
PA5-20577 can be used with blocking peptide PEP-0697.
Target Information
NENF is a secreted protein that is expressed in neurons but not glial cells of the brain. This protein has neurotrophic activity in primary cultured mouse neurons but not mitogenic activity in primary cultured mouse astrocytes. NENF activated mitogen-activated protein (MAP) and phosphotidylinositol-3 kinase pathways and could be inhibited by pertussis toxin but not receptor tyrosine kinases, suggesting that NENF acts through a Gi/Go-protein-coupled receptor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cell growth-inhibiting protein 47; Cell immortalization-related protein 2; nenf {ECO:0000250|UniProtKB:Q9CQ45}; Neudesin; neuron derived neurotrophic factor; Neuron-derived neurotrophic factor; Protein GIG47; SCIRP10-related protein; Secreted protein of unknown function; Spinal cord injury related protein 10; Spinal cord injury-related protein 10; SPUF protein
Gene Aliases: 1110060M21Rik; CIR2; NENF; SCIRP10; SPUF
UniProt ID: (Human) Q9UMX5, (Rat) Q6IUR5, (Mouse) Q9CQ45
Entrez Gene ID: (Human) 29937, (Rat) 289380, (Mouse) 66208
Molecular Function:
transmembrane signal receptor
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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