Immunofluorescent analysis of NF-E2 showing staining in the nucleus of HepG2 cells. HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a NF-E2 polyclonal antibody (Product # PA5-27762) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10µm.
|Tested species reactivity||Mouse, Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 1 and 373 of Human NF-E2|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 10% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ChIP assay (ChIP)||Assay-dependent|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-27762 targets NF-E2 in WB applications and shows reactivity with Human and mouse samples.
The PA5-27762 immunogen is recombinant fragment corresponding to a region within amino acids 1 and 373 of Human NF-E2.
Component of the NF-E2 complex essential for regulating erythroid and megakaryocytic maturation and differentiation. Binds to the hypersensitive site 2 (HS2) of the beta-globin control region (LCR). This subunit (NFE2) recognizes the TCAT/C sequence of the AP-1-like core palindrome present in a number of erythroid and megakaryocytic gene promoters. Requires MAFK or other small MAF proteins for binding to the NF-E2 motif. May play a role in all aspects of hemoglobin production from globin and heme synthesis to procurement of iron.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.