|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Rat, Mouse, Not Applicable|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Synthetic peptide corresponding to amino acids 526-539 (GLLSGDEDFSSIAD) of human NF-kB (p65) protein.|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||0.25-0.5 µg/10^6 cells|
|Immunohistochemistry (IHC)||5-15 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||5 µg/ml|
|Western Blot (WB)||2-5 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Suggested positive control: antigen standard for NFKB1 (transient overexpression lysate), Ramos, Daudi, HeLa, NIH-3T3.
NF-kB (nuclear factor kB) regulates the expression of a large number of genes that play critical roles in apoptosis, viral replication, tumorigenesis, various autoimmune diseases and inflammation. The active nuclear form of the NF-kB transcription factor complex is composed of two DNA binding subunits, NF-kB p65 and p50, both of which share extensive N-terminal sequence homology with the v-rel oncogene product. N-terminal regions of p50 and p65 are critical for DNA binding and help determine the DNA-binding specificities of p50 and p65.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Cardamonin reduces chemotherapy-enriched breast cancer stem-like cells in vitro and in vivo.
MA5-16160 was used in western blot to elucidate the reduction of chemotherap-enriched breast cancer stem-like cells in vivo and in vitro due to cardamonin
|Jia D,Tan Y,Liu H,Ooi S,Li L,Wright K,Bennett S,Addison CL,Wang L||Oncotarget (7:771)||2016|
Hepatoprotective effect of coenzyme Q10 in rats with acetaminophen toxicity.
MA5-16160 was used in immunohistochemistry to investigate the protective effect of coenzyme Q10 against acetaminophen toxicity in rat liver
|Fouad AA,Jresat I||Environmental toxicology and pharmacology (33:158)||2012|
Rutin attenuates cisplatin induced renal inflammation and apoptosis by reducing NF¿B, TNF-¿ and caspase-3 expression in wistar rats.
MA5-16160 was used in immunohistochemistry to investigate the mechanism for the protective effect of rutin on inflammation and cell death
|Arjumand W,Seth A,Sultana S||Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association (49:2013)||2011|
Protective effect of telmisartan against cadmium-induced nephrotoxicity in mice.
MA5-16160 was used in immunohistochemistry to investigate the protective effect of telmisartan against cadmium nephrotoxicity
|Fouad AA,Jresat I||Life sciences (89:29)||2011|
Ameliorative effects of telmisartan in diabetic rats with indomethacin-induced gastric ulceration.
MA5-16160 was used in immunohistochemistry to investigate the potential therapeutic application of telmisartan in diabetes treatment
|Fouad AA,Al-Sultan AI,Yacoubi MT,Gomaa W||European journal of pharmacology (637:162)||2010|
Coenzyme Q10 treatment ameliorates acute cisplatin nephrotoxicity in mice.
MA5-16160 was used in immunohistochemistry to investigate the protective effect of Coenzyme Q10 against cisplatin nephrotoxicity in mice
|Fouad AA,Al-Sultan AI,Refaie SM,Yacoubi MT||Toxicology (274:49)||2010|