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Invitrogen
NF2 Recombinant Rabbit Monoclonal Antibody (1H27L4)
Advanced Verification
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 5
NF2 Antibody (702466) in ICC/IF
For immunofluorescence analysis, DU-145 cells were fixed and permeabilized for detection of endogenous NF2 using Anti-NF2 Recombinant Rabbit Monoclonal Antibody (Product # 702466, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of NF2 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). ... View More
Product Details
702466
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
1H27L4
Immunogen
Protein corresponding to human NF2 [aa331-aa530]
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2688311
Product Specific Information
This antibody is predicted to react with Monkey, Pig, Horse, Mouse
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
NF2 encodes a protein, Merlin, that is similar to some members of the ERM family of proteins that are thought to link cytoskeletal components with proteins in the cell membrane. Merlin has been shown to interact with cell-surface proteins, proteins involved in cytoskeletal dynamics and proteins involved in regulating ion transport. This gene is expressed at high levels during embryonic development; in adults, significant expression is found in Schwann cells, meningeal cells, lens and nerve. Mutations in this gene are associated with neurofibromatosis type II which is characterized by nervous system and skin tumors and ocular abnormalities. Two predominant isoforms and a number of minor isoforms are produced by alternatively spliced transcripts.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Merlin; MGC123124; moesin-ezrin-radixin like; Moesin-ezrin-radixin-like protein; moesin-ezrin-radizin-like protein; neurofibromatosis 2; neurofibromin 2 (bilateral acoustic neuroma); neurofibromin 2 (merlin); Neurofibromin-2; OTTHUMP00000199607; Schwannomerlin; Schwannomin
Gene Aliases: ACN; BANF; merlin; Nf-2; NF2; SCH
UniProt ID: (Human) P35240, (Mouse) P46662, (Rat) Q63648
Entrez Gene ID: (Human) 4771, (Mouse) 18016, (Rat) 25744
mesoderm formation
negative regulation of cell-matrix adhesion
negative regulation of protein kinase activity
ectoderm development
negative regulation of DNA replication
negative regulation of cell proliferation
Schwann cell proliferation
regulation of gliogenesis
hippocampus development
negative regulation of cell-cell adhesion
actin cytoskeleton organization
negative regulation of cell growth
negative regulation of cell migration
regulation of protein stability
regulation of hippo signaling
odontogenesis of dentin-containing tooth
negative regulation of tyrosine phosphorylation of Stat3 protein
negative regulation of tyrosine phosphorylation of Stat5 protein
negative regulation of MAPK cascade
cell-cell junction organization
positive regulation of cell differentiation
negative regulation of JAK-STAT cascade
positive regulation of stress fiber assembly
lens fiber cell differentiation
regulation of stem cell proliferation
regulation of protein localization to nucleus
regulation of neural precursor cell proliferation
brain development
regulation of cell proliferation
regulation of neurogenesis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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