Western blot analysis of NF2/Merlin using anti-NF2/Merlin monoclonal antibody (MA5-17252) on (Lane 1) HeLa Cell lysate, (Lane 2) 293T Cell lysate, (Lane 3) MCF7 Cell lysate, (Lane 4) HepG2 Cell lysate, (Lane 5) MOLT4 Cell lysate, (Lane 6) C6 Cell lysate and (Lane 7) L292 Cell lysate.
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Recombinant human protein purified from E.coli (His-Merlin)|
|Storage buffer||HEPES with 0.15M NaCl, 0.01% BSA, 50% glycerol|
|Contains||0.03% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Western Blot (WB)||0.5 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
A suggested positive control for this product is 293T cells.
Probably acts as a membrane stabilizing protein. May inhibit PI3 kinase by binding to AGAP2 and impairing its stimulating activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.