Immunofluorescent analysis of NFAT1 showing staining in the cytoplasm of RAW264.7 cells. RAW264.7 cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a NFAT1 polyclonal antibody (Product # PA5-34940) diluted at 1:500. Blue: Hoechst 33342 staining.
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to a region within amino acids 1 and 99 of NFAT1|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-34940 targets NFAT1 in ICC, IF, and WB applications and shows reactivity with Human and Mouse samples.
The PA5-34940 immunogen is synthetic peptide corresponding to a region within amino acids 1 and 99 of NFAT1.
Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2, IL-3, IL-4, TNF-alpha or GM-CSF. Promotes invasive migration through the activation of GPC6 expression and WNT5A signaling pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.