Western blot of NFATC3/NFAT4 in Jurkat nuclear lysate was performed using 35ug protein in RIPA buffer. Lysates were probed with a NFATC3/NFAT4 polyclonal antibody (Product# PA5-37876) at a dilution of 0.03ug/ml and incubated for 1 hour, followed by chemiluminescent detection.
|Tested species reactivity||Human|
|Host / Isotype||Goat / IgG|
|Immunogen||Peptide with sequence C-HQELDAHEDDLQIN, from the internal region near the N-terminus of NFATC3|
|Purification||Antigen affinity chromatography|
|Storage buffer||TBS, pH 7.3, with 0.5% BSA|
|Contains||0.02% sodium azide|
|Tested Applications||Dilution *|
|Western Blot (WB)||0.03-0.1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with dog and cow based on sequence homology.
Acts as a regulator of transcriptional activation. Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.