Immunofluorescent analysis of NFATc1 using NFATc1 Monoclonal Antibody (7A6) (Product# MA3-024 ) shows staining in MCF-7 Cells. NFATc1 (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing NFATc1 (Product# MA3-024 ) at a dilution of 1:20 over night at 4 °C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.
|Tested species reactivity||Human, Mouse, Non-human primate, Rat|
|Published species reactivity||Rabbit, Rat, Hamster, Mouse, Human, Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Bacterially expressed glutathione S-transferase (GST) fusion protein containing NF-ATc1 residues 1-654.|
|Storage buffer||ascites diluted in PBS|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ChIP assay (ChIP)||Assay dependent|
|Gel Shift (GS)||Assay dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay Dependent|
|Immunohistochemistry (PFA fixed) (IHC (PFA))||Assay Dependent|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:2,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (Paraffin) (IHC (P))||See 1 publications below|
|Western Blot (WB)||See 21 publications below|
|ChIP assay (ChIP)||See 3 publications below|
|Immunocytochemistry (ICC)||See 5 publications below|
|Gel Shift (GS)||See 11 publications below|
|Immunoprecipitation (IP)||See 2 publications below|
|Immunohistochemistry (IHC)||See 3 publications below|
MA3-024 detects NFATc1 (NFAT2) from transfected human, rat, mouse, and monkey cells. This antibody does not cross react with NFATc2 (NFAT1).
MA3-024 has been successfully used in Western blot, immunoprecipitation, ChIP, immunofluorescence and gel shift procedures. By Western blot, this antibody detects an ~105 kDa band representing transfected NFAT2 from COS cell lysate.
The MA3-024 immunogen is bacterially expressed glutathione S-transferase (GST) fusion protein containing NF-ATc1 residues 1-654.
Antibodies to this protein (and modification) were previously sold as part of a Thermo Scientific Cellomics High Content Screening Kit. This replacement antibody is now recommended for researchers who need an antibody for high content cell-based assays. It has been thoroughly tested and validated for cellular immunofluorescence (IF) applications. Further optimization, including the selection of the most appropriate fluorescent Dylight conjugated secondary antibody, may have to be performed for your high content assay.
The product of this gene is a component of the nuclear factor of activated T cells DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation, and an inducible nuclear component. Proteins belonging to this family of transcription factors play a central role in inducible gene transcription during immune response. The product of this gene is an inducible nuclear component. It functions as a major molecular target for the immunosuppressive drugs such as cyclosporin A. Five transcript variants encoding distinct isoforms have been identified for this gene. Different isoforms of this protein may regulate inducible expression of different cytokine genes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
GDF11 decreases bone mass by stimulating osteoclastogenesis and inhibiting osteoblast differentiation.
MA3-024 was used in immunohistochemistry - paraffin section and western blot to study the role of GDF11 in bone remodeling
|Liu W,Zhou L,Zhou C,Zhang S,Jing J,Xie L,Sun N,Duan X,Jing W,Liang X,Zhao H,Ye L,Chen Q,Yuan Q||Nature communications (7:null)||2016|
Diaphragm assessment in mice overexpressing phospholamban in slow-twitch type I muscle fibers.
MA3-024 was used in western blot to study slow-twitch type 1 muscle fibers and diaphragm assessment in mice overexpressing phospholamban
|Fajardo VA,Smith IC,Bombardier E,Chambers PJ,Quadrilatero J,Tupling AR||Brain and behavior (6:null)||2016|
|Not Applicable||Not Cited||
Counter-regulation of T cell effector function by differentially activated p38.
MA3-024 was used in western blot to describe mechanisms of p38 activation that converge on NFATc1 and result in opposing effects on T cell immunity
|Alam MS,Gaida MM,Ogawa Y,Kolios AG,Lasitschka F,Ashwell JD||The Journal of experimental medicine (211:1257)||2014|
The effect of the degree of sulfation of glycosaminoglycans on osteoclast function and signaling pathways.
MA3-024 was used in western blot to study the effects of manufactured native and sulfated glycosaminoglycans on osteoblast function and signaling, and the significance for biomaterial use
|Salbach J,Kliemt S,Rauner M,Rachner TD,Goettsch C,Kalkhof S,von Bergen M,Möller S,Schnabelrauch M,Hintze V,Scharnweber D,Hofbauer LC||Biomaterials (33:8418)||2012|
STIM1-Ca(2+) signaling is required for the hypertrophic growth of skeletal muscle in mice.
MA3-024 was used in western blot to study hypertrophic murine skeletal muscle growth and the essential role of STIM1 regulated Ca(2+) signaling
|Li T,Finch EA,Graham V,Zhang ZS,Ding JD,Burch J,Oh-hora M,Rosenberg P||Molecular and cellular biology (32:3009)||2012|
Nuclear factor of activated T cells (NFAT) signaling regulates PTEN expression and intestinal cell differentiation.
MA3-024 was used in western blot to confirm the role of NFATc1 in PTEN expression and intestinal cell differentiation
|Wang Q,Zhou Y,Jackson LN,Johnson SM,Chow CW,Evers BM||Molecular biology of the cell (22:412)||2011|
Cyclin D1 is a bona fide target gene of NFATc1 and is sufficient in the mediation of injury-induced vascular wall remodeling.
MA3-024 was used in western blot to show the expression of NFATc1 is inhibited in HASMCs transfected with NFATc1 siRNA
|Karpurapu M,Wang D,Van Quyen D,Kim TK,Kundumani-Sridharan V,Pulusani S,Rao GN||The Journal of biological chemistry (285:3510)||2010|
Glycogen synthase kinase 3beta regulation of nuclear factor of activated T-cells isoform c1 in the vascular smooth muscle cell response to injury.
MA3-024 was used in western blot to study the effect of glycogen synthase kinase 3beta on NFATc1 in the vascular smooth muscle cell response to injury
|Chow W,Hou G,Bendeck MP||Experimental cell research (314:2919)||2008|
Increased oxidative properties of gastrocnemius in rats fed on a high-protein diet.
MA3-024 was used in western blot to investigate the effect of high-protein diet on the expression of NFATc1 in gastrocnemius muscles
|Nakazato K,Song H||The Journal of nutritional biochemistry (19:26)||2008|
Calcineurin-mediated slow-type fiber expression and growth in reloading condition.
MA3-024 was used in western blot to study the role of calcineurin in muscle growth and muscle type determination
|Miyazaki M,Hitomi Y,Kizaki T,Ohno H,Katsumura T,Haga S,Takemasa T||Medicine and science in sports and exercise (38:1065)||2006|
Defective IgG2a/2b class switching in PKC alpha-/- mice.
MA3-024 was used in western blot to study the effect of PKC alpha deletion on IgG2a/2b class switching
|Pfeifhofer C,Gruber T,Letschka T,Thuille N,Lutz-Nicoladoni C,Hermann-Kleiter N,Braun U,Leitges M,Baier G||Journal of immunology (Baltimore, Md. : 1950) (176:6004)||2006|
2-Arachidonoyl-glycerol suppresses interferon-gamma production in phorbol ester/ionomycin-activated mouse splenocytes independent of CB1 or CB2.
MA3-024 was used in western blot to study the effect of 2-arachidonoyl-glycerol on interferon-gamma in mouse splenocytes
|Kaplan BL,Ouyang Y,Rockwell CE,Rao GK,Kaminski NE||Journal of leukocyte biology (77:966)||2005|
Blockade of nuclear factor of activated T cells activation signaling suppresses balloon injury-induced neointima formation in a rat carotid artery model.
MA3-024 was used in western blot to demonstrate the role of nuclear factor of activated T cells in neointima formation.
|Liu Z,Zhang C,Dronadula N,Li Q,Rao GN||The Journal of biological chemistry (280:14700)||2005|
Hypertrophy and transcriptional regulation induced in myogenic cell line L6-C5 by an increase of extracellular calcium.
MA3-024 was used in western blot to investigate the effect of calcium concentration on cell growth and gene expresion in myogenic cell line L6-C5
|De Arcangelis V,Coletti D,Canato M,Molinaro M,Adamo S,Reggiani C,Naro F||Journal of cellular physiology (202:787)||2005|
Contribution of the calcineurin signaling pathway to overload-induced skeletal muscle fiber-type transition.
MA3-024 was used in western blot to study the role of the calcineurin signaling pathway during overload-induced skeletal muscle fiber-type transition
|Miyazaki M,Hitomi Y,Kizaki T,Ohno H,Haga S,Takemasa T||Journal of physiology and pharmacology : an official journal of the Polish Physiological Society (55:751)||2004|
Differential roles for Wiskott-Aldrich syndrome protein in immune synapse formation and IL-2 production.
MA3-024 was used in western blot to investigate the role of Wiskott-Aldrich syndrome protein in immune synapse formation and IL-2 production
|Cannon JL,Burkhardt JK||Journal of immunology (Baltimore, Md. : 1950) (173:1658)||2004|
TRPC3 channels confer cellular memory of recent neuromuscular activity.
MA3-024 was used in western blot to study the regulation of NFAT functions through neuromuscular stimulation in rodent skeletal muscles.
|Rosenberg P,Hawkins A,Stiber J,Shelton JM,Hutcheson K,Bassel-Duby R,Shin DM,Yan Z,Williams RS||Proceedings of the National Academy of Sciences of the United States of America (101:9387)||2004|
Protein kinase C theta affects Ca2+ mobilization and NFAT cell activation in primary mouse T cells.
MA3-024 was used in western blot to study the role of protein kinase C theta in calcium mobilization and NFAT activation in mouse T cells.
|Pfeifhofer C,Kofler K,Gruber T,Tabrizi NG,Lutz C,Maly K,Leitges M,Baier G||The Journal of experimental medicine (197:1525)||2003|
Caspase-mediated calcineurin activation contributes to IL-2 release during T cell activation.
MA3-024 was used in western blot to study the role of NFATc in interleukin-2 transactivation
|Mukerjee N,McGinnis KM,Gnegy ME,Wang KK||Biochemical and biophysical research communications (285:1192)||2001|
The calcineurin-NFAT pathway and muscle fiber-type gene expression.
MA3-024 was used in western blot to investigate the role of calcineurin-NFAT pathway in the regulation of muscle fiber-type gene expression
|Swoap SJ,Hunter RB,Stevenson EJ,Felton HM,Kansagra NV,Lang JM,Esser KA,Kandarian SC||American journal of physiology. Cell physiology (279:C915)||2000|
Histamine induces nuclear factor of activated T cell-mediated transcription and cyclosporin A-sensitive interleukin-8 mRNA expression in human umbilical vein endothelial cells.
MA3-024 was used in western blot to study the roles of histamine during NFAT-mediated transcription
|Boss V,Wang X,Koppelman LF,Xu K,Murphy TJ||Molecular pharmacology (54:264)||1998|
The cyclosporin A-sensitive nuclear factor of activated T cells (NFAT) proteins are expressed in vascular smooth muscle cells. Differential localization of NFAT isoforms and induction of NFAT-mediated transcription by phospholipase C-coupled cell surface receptors.
MA3-024 was used in western blot to demonstrate the differential localization of NFAT isoforms expressed in vascular smooth muscle cells
|Boss V,Abbott KL,Wang XF,Pavlath GK,Murphy TJ||The Journal of biological chemistry (273:19664)||1998|
|Not Applicable||Not Cited||
Identification of novel regulatory NFAT and TFII-I binding elements in the calbindin-D28k promoter in response to serum deprivation.
MA3-024 was used in ChIP assay to identify novel components for regulation called NFAT and TFII-I binding elements in the calbindin-D28k promoter in response to serum deprivation
|Hajibeigi A,Dioum EM,Guo J,Öz OK||Biochemical and biophysical research communications (465:414)||2015|
Novel interactions between NFATc1 (Nuclear Factor of Activated T cells c1) and STAT-3 (Signal Transducer and Activator of Transcription-3) mediate G protein-coupled receptor agonist, thrombin-induced biphasic expression of cyclin D1, with first phase influencing cell migration and second phase directing cell proliferation.
MA3-024 was used in ChIP assay to study the role of NFATc1 and STAT3 in mediating the expression of cyclin D1
|Kundumani-Sridharan V,Van Quyen D,Subramani J,Singh NK,Chin YE,Rao GN||The Journal of biological chemistry (287:22463)||2012|
FOXP3 inhibits activation-induced NFAT2 expression in T cells thereby limiting effector cytokine expression.
MA3-024 was used in chromatin immunoprecipitation to study the effect of FOXP3 on NFAT-mediated inflammatory gene expression.
|Torgerson TR,Genin A,Chen C,Zhang M,Zhou B,Añover-Sombke S,Frank MB,Dozmorov I,Ocheltree E,Kulmala P,Centola M,Ochs HD,Wells AD,Cron RQ||Journal of immunology (Baltimore, Md. : 1950) (183:907)||2009|
Nuclear factor of activated T cells c1 mediates p21-activated kinase 1 activation in the modulation of chemokine-induced human aortic smooth muscle cell F-actin stress fiber formation, migration, and proliferation and injury-induced vascular wall remodeling.
MA3-024 was used in immunocytochemistry and western blot to study the roles of NFATc1 and Pak1 in chemokine-induced aortic smooth muscle proliferation and vascular remodeling following injury
|Kundumani-Sridharan V,Singh NK,Kumar S,Gadepalli R,Rao GN||The Journal of biological chemistry (288:22150)||2013|
Cyclosporine A impairs nucleotide binding oligomerization domain (Nod1)-mediated innate antibacterial renal defenses in mice and human transplant recipients.
MA3-024 was used in immunocytochemistry to study the downregulation of Nod1 expression by cyclosprine A in a murine model of acute pyelonephritis and in renal transplant patients
|Tourneur E,Ben Mkaddem S,Chassin C,Bens M,Goujon JM,Charles N,Pellefigues C,Aloulou M,Hertig A,Monteiro RC,Girardin SE,Philpott DJ,Rondeau E,Elbim C,Werts C,Vandewalle A||PLoS pathogens (9:null)||2013|
Activity- and calcineurin-independent nuclear shuttling of NFATc1, but not NFATc3, in adult skeletal muscle fibers.
MA3-024 was used in immunocytochemistry to investigate the nucleocytoplasmic shuttling of NFATc1.
|Shen T,Liu Y,Cseresnyés Z,Hawkins A,Randall WR,Schneider MF||Molecular biology of the cell (17:1570)||2006|
Activation of the Ca(2+)/calcineurin/NFAT2 pathway controls smooth muscle cell differentiation.
MA3-024 was used in immunocytochemistry and western blot to study the role of Ca(2+)/calcineurin/NFAT2 pathway activation in regulating smooth muscle cell differentiation
|Larrieu D,Thiébaud P,Duplàa C,Sibon I,Thézé N,Lamazière JM||Experimental cell research (310:166)||2005|
c-Jun NH(2)-terminal kinase inhibits targeting of the protein phosphatase calcineurin to NFATc1.
MA3-024 was used in immunocytochemistry to examine the role of JNK1 in NFATc1 regulation.
|Chow CW,Dong C,Flavell RA,Davis RJ||Molecular and cellular biology (20:5227)||2000|
Nuclear orphan receptor NR2F6 directly antagonizes NFAT and ROR¿t binding to the Il17a promoter.
ma3-024 was used in EMSA to study the role of the NR2F6 orphan nuclear receptor in modulating Il17a transcription by antagonizing the promoter binding of RORgamma-t and NFAT
|Hermann-Kleiter N,Meisel M,Fresser F,Thuille N,Müller M,Roth L,Katopodis A,Baier G||Journal of autoimmunity (39:428)||2012|
NFATc1 regulation of TRAIL expression in human intestinal cells.
MA3-024 was used in EMSA to investigate the regulatory effect of NFATc1 on TRAIL in human intestinal cells
|Wang Q,Zhou Y,Weiss HL,Chow CW,Evers BM||PloS one (6:null)||2011|
Calcium-dependent activation of interleukin-21 gene expression in T cells.
MA3-024 was used in EMSA to demonstrate the induction of the IL-21 in preactivated T lymphocytes by a calcium signal.
|Kim HP,Korn LL,Gamero AM,Leonard WJ||The Journal of biological chemistry (280:25291)||2005|
A potential role for nuclear factor of activated T-cells in receptor tyrosine kinase and G-protein-coupled receptor agonist-induced cell proliferation.
MA3-024 was used in EMSA to investigate the role of NFATs in receptor tyrosine kinase and GRCR agonist-induced proliferation of vascular smooth muscle cells
|Yellaturu CR,Ghosh SK,Rao RK,Jennings LK,Hassid A,Rao GN||The Biochemical journal (368:183)||2002|
Cannabinol enhancement of interleukin-2 (IL-2) expression by T cells is associated with an increase in IL-2 distal nuclear factor of activated T cell activity.
MA3-024 was used in EMSA assay to investigate the mechanism of cannabinol enhancement of interleukin-2 (IL-2) expression by CBN in T cells
|Jan TR,Rao GK,Kaminski NE||Molecular pharmacology (61:446)||2002|
Double-stranded RNA regulates IL-4 expression.
MA3-024 was used in EMSA to investigate the role of the low concentrations of dsRNA towards the IL-4 expression.
|Kehoe KE,Brown MA,Imani F||Journal of immunology (Baltimore, Md. : 1950) (167:2496)||2001|
Evidence for suppressed activity of the transcription factor NFAT1 at its proximal binding element P0 in the IL-4 promoter associated with enhanced IL-4 gene transcription in T cells of atopic patients.
MA3-024 was used in EMSA assay to study why IL-4 gene is upregulated in atopy
|Wierenga EA,Walchner M,Kick G,Kapsenberg ML,Weiss EH,Messer G||International immunology (11:297)||1999|
Involvement of Jun and Rel proteins in up-regulation of interleukin-4 gene activity by the T cell accessory molecule CD28.
MA3-024 was used in EMSA assay to study the mechanism for the effect of CD28 on interleukin-4 gene function
|Li-Weber M,Giasi M,Krammer PH||The Journal of biological chemistry (273:32460)||1998|
Th2-specific protein/DNA interactions at the proximal nuclear factor-AT site contribute to the functional activity of the human IL-4 promoter.
MA3-024 was used in EMSA assay to study the mechanism for the function of human IL-4
|Li-Weber M,Salgame P,Hu C,Davydov IV,Laur O,Klevenz S,Krammer PH||Journal of immunology (Baltimore, Md. : 1950) (161:1380)||1998|
Two NFAT transcription factor binding sites participate in the regulation of CD95 (Fas) ligand expression in activated human T cells.
MA3-024 was used in EMSA assay to investigate the regulation of CD95 expression by NFAT
|Latinis KM,Norian LA,Eliason SL,Koretzky GA||The Journal of biological chemistry (272:31427)||1997|
Expression of NFAT-family proteins in normal human T cells.
MA3-024 was used in EMSA assay to investigate the expression levels of various NFAT proteins in normal human T cells
|Lyakh L,Ghosh P,Rice NR||Molecular and cellular biology (17:2475)||1997|
The amiloride derivative phenamil attenuates pulmonary vascular remodeling by activating NFAT and the bone morphogenetic protein signaling pathway.
MA3-024 was used in immunoprecipitation to investigate the effect of constitutive elevation of Trb3 on IPAH and FPAH therapy
|Chan MC,Weisman AS,Kang H,Nguyen PH,Hickman T,Mecker SV,Hill NS,Lagna G,Hata A||Molecular and cellular biology (31:517)||2011|
The CCAAT/enhancer binding protein beta (C/EBPbeta) cooperates with NFAT to control expression of the calcineurin regulatory protein RCAN1-4.
MA3-024 was used in immunoprecipitation to investigate the role of the CCAAT/enhancer binding protein beta during calcineurin/NFAT activation of RCAN1-4 expression
|Oh M,Dey A,Gerard RD,Hill JA,Rothermel BA||The Journal of biological chemistry (285:16623)||2010|
NFAT is a nerve activity sensor in skeletal muscle and controls activity-dependent myosin switching.
MA3-024 was used in immunohistochemistry to study the role of NFAT in activity-dependent fiber type specification in skeletal muscle.
|McCullagh KJ,Calabria E,Pallafacchina G,Ciciliot S,Serrano AL,Argentini C,Kalhovde JM,Lømo T,Schiaffino S||Proceedings of the National Academy of Sciences of the United States of America (101:10590)||2004|
Maintenance of muscle mass is not dependent on the calcineurin-NFAT pathway.
MA3-024 was used in immunohistochemistry to study the role of the calcineurin-NFAT pathway in the maintenance of muscle mass
|Dupont-Versteegden EE,Knox M,Gurley CM,Houlé JD,Peterson CA||American journal of physiology. Cell physiology (282:C1387)||2002|
Nuclear accumulation of NFAT4 opposed by the JNK signal transduction pathway.
MA3-024 was used in immunohistochemistry to demonstrate that nuclear accumulation of NFAT4 is regulated by JNK
|Chow CW,Rincón M,Cavanagh J,Dickens M,Davis RJ||Science (New York, N.Y.) (278:1638)||1997|