|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment contain a sequence corresponding to a region within amino acids 322 and 969 of NFkB p105/p50|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-27266 targets NFkB p105 / p50 in WB applications and shows reactivity with Human samples.
The PA5-27266 immunogen is a recombinant fragment corresponding to a region within amino acids 322 and 969 of NFkB p105/p50.
This gene encodes a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Nucleoside, nucleotide and nucleic acid metabolism mRNA transcription mRNA transcription regulation Signal transduction Intracellular signaling cascade NF-kappaB cascade Immunity and defense B-cell- and antibody-mediated immunity Developmental processes Apoptosis Inhibition of apoptosis