Antibody target was verified by Relative expression to ensure the antibody binds to the antigen stated. View details.
|ChIP assay (ChIP)||1-5 µg x 10^6 cells|
|Immunocytochemistry (ICC)||1-2 ug/ml|
|Immunofluorescence (IF)||1-2 ug/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20-1:200|
|Western Blot (WB)||2-3 ug/ml|
|Tested Species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Peptide corresponding to amino acids 143–156 of human p50|
|Contains||0.09% sodium azide|
|Storage conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale and purified with Protein A.
ABfinity™ oligoclonal antibodies comprise a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds—the sensitivity of a polyclonal antibody with the specificity of a monoclonal, all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody—recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets when compared with monoclonal antibodies—an oligoclonal antibody has a known mixture of light and heavy chains. This exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
The p50 protein is a member of the NF- kappaB/Rel transcription factor family that is a subunit of the NF- kappaB protein complex. p50 is processed from a 105 kDa precursor protein by the 26S proteasome, and can exist as either a heterodimer or a homodimer. Heterodimers formed with Rel proteins activate transcription, but because p50 lacks a C terminal transactivation domain, the homodimer acts as a repressor unless it associates with a co-activator such as BCL3. The NF- kappaB protein complex expressed in most cell types, and is primarily found in the cytoplasm in an inactive state in association with I-kappa-B. Phosphorylation of I-kappa-B releases the complex, allowing translocation to the nucleus and modification of gene expression. Phosphorylation of the p50 subunit at the Ser-337 residue has been shown to significantly increase DNA binding efficiency of the NF- kappaB protein complex.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: DNA binding factor KBF1; DNA-binding factor KBF1; EBP-1; NF kappaB1; NF-kappa-B1 p84/NF-kappa-B1 p98; NF-kappaB p50; NF-kappabeta; nuclear factor kappa-B DNA binding subunit; nuclear factor kappaB p50; Nuclear factor NF-kappa-B p105 subunit; nuclear factor NF-kappa-B p50 subunit; nuclear factor of kappa light chain gene enhancer in B-cells 1, p105; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1; nuclear factor of kappa light polypeptide gene enhancer in B-cells 1, p105; nuclear factor-kappaB p50; OTTHUMP00000219572; p50 subunit of NF kappaB; p50 subunit of NF-kappaB
Gene Aliases: CVID12; EBP-1; KBF1; NF-kappa-B; NF-kappaB; NF-kappaB1; NF-KB1; NFkappaB; NFKB-p105; NFKB-p50; NFKB1; p105; p50; p50/p105
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