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Invitrogen

NFkB p50/p105 Polyclonal Antibody, eBioscience™

3 Published Figures
10 References
View all (38) NFkB p50/p105 antibodies

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Product Details

14-6732-81

Applications
Tested Dilution
Publications

Western Blot (WB)

1:400
View 7 publications 7 publications

Immunohistochemistry (IHC)

Assay-Dependent
View 1 publication 1 publication

Immunoprecipitation (IP)

Assay-Dependent
-

Gel Shift (EMSA)

-
View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-
View 1 publication 1 publication
Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Amount

50 µg

Purification

Affinity chromatography

Storage buffer

PBS with BSA

Contains

0.1% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_468257

Product Specific Information

Description: The polyclonal antibody reacts with mouse, rat, and human NFkB p50; the antibody was raised against a peptide mapping to the carboxy terminus of human NFkB p50. Members of the rel/NFkB family of transcription factors are involved in the regulation of cellular responses, such as growth, development, and the inflammatory response. They share a structural motif known as the rel homology region (RHR), the C-terminal one third of which mediates protein dimerization (2, 6, 8). Complexes of p50 (NF-kB1) or p52 (NF-kB2) are generated through the processing of p105 and p100 precursors, respectively. These are usually associated with members of the Rel family (p65, c-Rel, Rel B). The homo- and heterodimer formed through combinations of NFkB/Rel proteins bind distinct kB sites to regulate the transcription of different genes (7, 9). In resting cells, NFkB is retained in the cytoplasm bound to inhibitory proteins of the IkB family. Degradation of IkB proteins occurs with cell activation, via of variety of signals, including inflammatory cytokines and bacterial lipopolysaccharides (LPS) as well as oxidative and fluid mechanical stress. This results in nuclear translocation of NFkB and the transcriptional gene activation of proinflammatory genes (1, 9). It has been suggested that NFkB plays a role in the development of numerous pathological states. Activation of NFkB induces gene programs leading to transcription of factors that promote inflammation, such as leukocyte adhesion molecules, cytokines, and chemokines. It is also thought that there are some substances with possible anti-inflammatory effects that are also NFkB regulated. There is some evidence indicating NFkB as a key factor in the pathophysiology of cardiac ischemia-reperfusion injury as well as the development of insulin dependent Diabetes Mellitus (4, 3).

Applications Reported: Purified anti-mo/hu/rat NFkB p50 and p105 poly has been reported for use in immunoprecipitation, immunoblotting (WB), and immunohistochemical staining.

Applications Tested: Purified anti-mo/hu/rat NFkB p50 and p105 poly has been tested by immunoblotting (WB). (1:400 starting dilution). It is recommended that this antibody be titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

NF-kB is a nuclear transcription factor activated by various extra and intracellular stimuli such as cytokines, UV radiation, stress, in injury, and by bacterial and viral products. It is involved in regulation of various cellular events including cell growth, differentiation, proliferation, apoptosis and inflammation. NFKB1 (p50), a 50KDa functional sub-unit of NF-kB, is a member of Rel protein family. It is synthesized as a p105 precursor protein and consists of an N-terminal conserved RHD-region containing nuclear localization signal, DNA-binding and dimerization domains. NFKB1 (p50) forms homodimers or heterodimerizes with p65, forming the functional NF-kB factor. NFKB1 (p50) directs the nuclear translocation of NF-kB and is instrumental in its DNA-bidning. Pathological role of NF-kB has been suggested in AIDS, hematogenic cancer cell metastasis and rheumatoid arthritis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: DKFZp686C01211; DNA-binding factor KBF1; EBP-1; MGC54151; NF kappaB1; NF KB; NF-kappa-B1 p84/NF-kappa-B1 p98; NF-kappaB p50; NF-kB p50/p105; nuclear factor; nuclear factor kappa b; nuclear factor kappa-B DNA binding subunit; nuclear factor kappaB p50; nuclear factor NF-kappa-B p50; nuclear factor NF-kappa-B p50 subunit; nuclear factor of kappa light chain gene enhancer in B-cells 1, p105; nuclear factor of kappa light polypeptide gene enhancer in B-cells 1; nuclear factor of kappa light polypeptide gene enhancer in B-cells 1, p105; p105; p50 subunit of NF kappaB; p50/p105; transcription factor p105 NF-kappa B precursor; unnamed protein product

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Gene Aliases: CVID12; EBP-1; KBF1; NF-kappa-B1; NF-kappaB; NF-kappaB1; NF-kappabeta; NF-kB; NF-KB1; NFkappaB; NFKB-p105; NFKB-p50; p105; p50; p50/p105

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UniProt ID: (Human) P19838, (Mouse) P25799, (Rat) Q63369

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Entrez Gene ID: (Human) 4790, (Mouse) 18033, (Rat) 81736

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Function(s)
transcription regulatory region sequence-specific DNA binding RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding core promoter proximal region sequence-specific DNA binding transcription coactivator binding transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding transcription cofactor activity protein binding identical protein binding actinin binding sequence-specific DNA binding protein sequestering activity double-stranded DNA binding heat shock protein binding macromolecular complex binding sequence-specific double-stranded DNA binding
Process(es)
negative regulation of transcription from RNA polymerase II promoter MAPK cascade negative regulation of cytokine production regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter inflammatory response signal transduction I-kappaB kinase/NF-kappaB signaling JNK cascade gene expression regulation of gene expression negative regulation of gene expression positive regulation of macrophage derived foam cell differentiation positive regulation of cholesterol efflux positive regulation of lipid storage negative regulation of vitamin D biosynthetic process tumor necrosis factor-mediated signaling pathway NIK/NF-kappaB signaling negative regulation of apoptotic process positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter negative regulation of inflammatory response B cell receptor signaling pathway mammary gland involution positive regulation of transcription initiation from RNA polymerase II promoter cellular response to lipopolysaccharide cellular response to mechanical stimulus cellular response to nicotine cellular response to interleukin-6 cellular response to tumor necrosis factor positive regulation of canonical Wnt signaling pathway cellular response to interleukin-17 cellular response to virus antibacterial innate immune response negative regulation of cytokine production involved in inflammatory response positive regulation of hyaluronan biosynthetic process cellular response to angiotensin positive regulation of miRNA metabolic process response to oxidative stress positive regulation of gene expression signal transduction involved in regulation of gene expression negative regulation of interleukin-12 production response to muscle stretch negative regulation of transcription, DNA-templated lymph node development positive regulation of inflammatory response cellular response to carbohydrate stimulus cellular response to cytokine stimulus cellular response to interleukin-1 cellular response to dsRNA cellular response to peptide cellular response to diterpene cellular response to glucoside cellular response to brain-derived neurotrophic factor stimulus response to xenobiotic stimulus response to bacterium response to ethanol response to copper ion
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