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NF-kappa-B (p65) Clone: 572 Cat. No. 436700. Immunoflorescence of 4% PFA-fixed Hela cells (A) NF-kappa-B (P65) Clone: 572 Cat. No. 436700 shown in green, Alexa Fluor® 488 secondary antibody. Nuclei are shown in blue with DAPI. (B) No primary antibody (negative control), Alexa Fluor® 488 secondary antibody, and Nuclei are shown in blue with DAPI.
|Tested species reactivity||Bovine, Dog, Horse, Human, Mouse, Non-human primate, Rhesus monkey, Rat|
|Published species reactivity||Human, Mouse|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Recombinant protein derived from the N-terminus region of human NFkB (p65) protein|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||2-3 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
NF-kappaB (Nuclear Factor kappa B) is a nuclear transcription factor found in all cell types and is involved in cellular responses to stimuli such as stress, cytokines, free radicals, ultraviolet irradiation, and bacterial or viral antigens. NF-kappaB plays a key role in regulating the immune response to infection. Consistent with this role, incorrect regulation of NF-kappaB has been linked to cancer, inflammatory and autoimmune diseases, septic shock, viral infection and improper immune development. There are five members in the NF-kappaB family: NF-kappaB1, NF-kappaB2, RelA (also named p65), RelB, and c-Rel. The most common form of NF-kappaB is the p50/RelA heterodimer, although other forms of NF-kappaB dimers, such as p50/p50, p52/p52, p52/RelA, p50/c-Rel, c-Rel/c-Rel, p52/RelB, and p50/RelB, have also been identified in some types of cells. The primary role of NF-kappaB is to maintain normal cellular functions that range from cell-to-cell communication to cell motility, cell cycle progression, and cell lineage development. The activity of NF-kappaB is tightly regulated by interaction with inhibitory IkappaB proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Possible Mechanisms of Di(2-ethylhexyl) Phthalate-Induced MMP-2 and MMP-9 Expression in A7r5 Rat Vascular Smooth Muscle Cells.
436700 was used in western blot to test if DEHP affects MMP-2 or MMP-9 expression in vascular smooth muscle cells
|Shih MF,Pan KH,Cherng JY||International journal of molecular sciences (16:28800)||2015|
Co-expression of ß-arrestin1 and NF-¿B is associated with cancer progression and poor prognosis in lung adenocarcinoma.
436700 was used in immunohistochemistry (paraffin) to correlate β-arrestin1 and NF-κB expression and the clinicopathological characteristics in lung adenocarcinoma.
|Yu J,Wang L,Zhang T,Shen H,Dong W,Ni Y,Du J||Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine (36:6551)||2015|
Memory and PTPIP51--a new protein in hippocampus and cerebellum.
436700 was used in proximity ligation assay to study the role of PTPIP51 in memory processing.
|Brobeil A,Viard M,Petri MK,Steger K,Tag C,Wimmer M||Molecular and cellular neurosciences (64:61)||2015|
Targeting I¿B proteins for HIV latency activation: the role of individual I¿B and NF-¿B proteins.
436700 was used in western blot to study the role of individual IkappaB and NF-kappaB proteins for HIV latency activation
|Fernandez G,Zaikos TD,Khan SZ,Jacobi AM,Behlke MA,Zeichner SL||Journal of virology (87:3966)||2013|
Tumor protein p63/nuclear factor ¿B feedback loop in regulation of cell death.
436700 was used in western blot to elucidate the functional relationship between TP63 isotypes and NF-κB.
|Sen T,Sen N,Huang Y,Sinha D,Luo ZG,Ratovitski EA,Sidransky D||The Journal of biological chemistry (286:43204)||2011|
avian reticuloendotheliosis viral (v-rel) oncogene homolog A; NF-kappa-B p65delta3; NF-kappa-B transcription factor p65; NFKB3; Nuclear Factor kB p65 subunit; Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3; p65 NF kappaB; p65 NF-kappa B; p65 NFkB; RelA; Transcription Factor p65; v-rel avian reticuloendotheliosis viral oncogene homolog A; v-rel reticuloendotheliosis viral oncogene homolog A
NFKB3; p65; RELA