Western blot of NFkB p65 in whole extract of NIH-3T3 cells using a NFkB p65 monoclonal antibody (Product # MA1-24691) at a dilution of 1:250 (lane 1) 1:500 (lane 2) and 1:1000 (lane 3) followed by detection using a Goat anti-mouse IgG-Peroxidase and chemiluminescent substrate.
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Recombinant mouse NF-kB p65 fragment (C-terminal 151 aa)|
|Contains||15mM sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-24691 detects NFkB p65 from human and mouse samples.
MA1-24691 has been successfully used in Western blot procedures.
The MA1-24691 immunogen is a recombinant mouse NF-kB p65 fragment (C-terminal 151 aa).
Positive Control: 3T3 whole cell extract
Store at 4°C for short term. For extended storage aliquot and store at -20°C or below. Avoid Freeze/Thaw Cycles.
The transcription factor NFkB is widely recognized as a critical mediator of immune and inflammatory responses. In most cell types, NFkB is found in the cytoplasm where it is associated with an inhibitory protein known as IkB. An impressive variety of stimuli (tumor necrosis factor, interleukin 1, T-cell activation signals, bacterial endotoxins, viral transforming proteins, certain growth factors and reactive oxygen intermediates) lead to the rapid nuclear assumulation of NFkB by the induced phosphorylation and subsquent degradation of IkB. In the nucleus, NFkB regulates genes encoding cytokines, cytokine receptors, cell adhesion molecules, proteins involved in coagulation and genes involved in cell growth control. Additionally, NFkB is thought to be an important transcriptional regulator for HIV. Growing evidence indicates that the dysregulation of NFkB may be key to a number of diseases including arthritis and other inflammatory diseases, Alzheimer's disease, atherosclerosis, and cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.