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      View (42) other NFkB p65 antibodies

      Invitrogen

      NFkB p65 Antibody (4-2HCLC), ABfinity™ Rabbit Oligoclonal

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      Antibody target was verified by KnockOut to ensure the antibody binds to the antigen stated. View details.

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      Catalog #:710048

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      Product Details

      TESTED APPLICATIONS DILUTION
      ChIP assay (ChIP) 3µg
      ELISA (ELISA) Assay-Dependent
      Immunocytochemistry (ICC) 1µg/ml
      Immunofluorescence (IF) 1µg/ml
      Immunohistochemistry (Paraffin) (IHC (P)) 1:10-1:100
      Western Blot (WB) 0.5-1µg/ml
      Tested Species reactivity Human, Mouse
      Host / Isotype Rabbit / IgG
      Class Oligoclonal
      Type Antibody
      Clone 4-2HCLC
      Immunogen Recombinant protein corresponding to amino acids 375-551 of human NF kappaB p65.
      Conjugate Unconjugated
      Form Liquid
      Concentration 0.5 mg/ml
      Purification Protein A
      Storage buffer PBS
      Contains 0.09% sodium azide
      Storage conditions Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C
      RRID AB_2532539

      Product Specific Information

      ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale and purified with Protein A.

      ABfinity™ oligoclonal antibodies comprise a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds—the sensitivity of a polyclonal antibody with the specificity of a monoclonal, all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody—recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets when compared with monoclonal antibodies—an oligoclonal antibody has a known mixture of light and heavy chains. This exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.

      Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.

      Background/Target Information

      NF kappaB p65 belongs to the Rel family of transcription factors. NF kappaB homo and heterodimers are retained in an inert, non-DNA-binding form in the cytosol by inhibitory molecules in most cells. Activation of NF kappaB occurs predominantly through the phosphorylation and degradation of I kappaB proteins. The free NF kappaB translocates to the nucleus, where it upregulates expression from genes involved in developmental processes, cellular growth, apoptosis, and immune and inflammatory responses. In humans, the gene is located on the q arm of chromosome 11.

      For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

      Bioinformatics

      Protein Aliases: avian reticuloendotheliosis viral (v-rel) oncogene homolog A; NF-kappa-B p65delta3; NF-kappa-B transcription factor p65; Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3; p65 NF kappaB; p65 NF-kappa B; p65 NFkB; Transcription factor p65; v-rel avian reticuloendotheliosis viral oncogene homolog A; v-rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 (p65)); v-rel reticuloendotheliosis viral oncogene homolog A

      Gene Aliases: NFKB3; p65; RELA

      UniProt ID: (Human) Q04206, (Mouse) Q04207

      Entrez Gene ID: (Human) 5970, (Mouse) 19697

      Molecular Function: nucleic acid binding transcription factor

      Function(s) Blue Arrow Icon
      negative regulation of transcription from RNA polymerase II promoter liver development hair follicle development stimulatory C-type lectin receptor signaling pathway acetaldehyde metabolic process transcription from RNA polymerase II promoter inflammatory response cellular defense response aging positive regulation of cell proliferation organ morphogenesis response to organic substance response to UV-B positive regulation of Schwann cell differentiation cytokine-mediated signaling pathway membrane protein intracellular domain proteolysis positive regulation of chondrocyte differentiation positive regulation of type I interferon production response to muramyl dipeptide response to progesterone response to insulin response to cobalamin cellular response to hepatocyte growth factor stimulus response to muscle stretch Fc-epsilon receptor signaling pathway negative regulation of protein catabolic process response to drug negative regulation of apoptotic process positive regulation of I-kappaB kinase/NF-kappaB signaling response to amino acid response to morphine positive regulation of interleukin-12 biosynthetic process negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter negative regulation of insulin receptor signaling pathway regulation of inflammatory response T cell receptor signaling pathway positive regulation of NF-kappaB transcription factor activity response to cAMP defense response to virus cellular response to hydrogen peroxide nucleotide-binding oligomerization domain containing 2 signaling pathway response to interleukin-1 cellular response to lipopolysaccharide cellular response to nicotine cellular response to interleukin-1 cellular response to interleukin-6 cellular response to tumor necrosis factor cellular response to peptide hormone stimulus negative regulation of NIK/NF-kappaB signaling positive regulation of miRNA metabolic process negative regulation of extrinsic apoptotic signaling pathway transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter defense response I-kappaB kinase/NF-kappaB signaling response to bacterium response to cytokine NIK/NF-kappaB signaling innate immune response
      Process(es) Blue Arrow Icon
      RNA polymerase II regulatory region sequence-specific DNA binding
       RNA polymerase II core promoter proximal region sequence-specific DNA binding
       RNA polymerase II distal enhancer sequence-specific DNA binding
       transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
       transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
       transcriptional activator activity, RNA polymerase II distal enhancer sequence-specific binding
       DNA binding
       chromatin binding
       transcription factor activity, sequence-specific DNA binding
       transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding
       protein binding
       transcription factor binding
       protein kinase binding
       chromatin DNA binding
       ubiquitin protein ligase binding
       protein complex binding
       activating transcription factor binding
       phosphate ion binding
       identical protein binding
       protein homodimerization activity
       actinin binding
       histone deacetylase binding
       transcription regulatory region DNA binding
       protein heterodimerization activity
       protein N-terminus binding
       NF-kappaB binding
       repressing transcription factor binding
       ankyrin repeat binding
       enzyme binding
       sequence-specific DNA binding

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