This Antibody was verified by KnockDown to ensure that the antibody binds to the antigen stated. View Details
PA5-16545 targets NFkappaB/p65 in IHC (P), IF, and WB applications and shows reactivity with mouse, Rabbit, Rat, Xn, Bovine, Canine, Drosophila, Hamster, Human, and Porcine samples.
The PA5-16545 immunogen is a synthetic peptide from the C-terminus of human NFkappaB protein.
This antibody was orginally validated as part of a Thermo Scientific Cellomics High Content Screening Kit. The antibody sold separately may have slightly different performance and may need to be further optimized for the best results.
NFkappa B is a heterodimer consisting of a 50kDa DNA binding subunit and a 65kDa transactivating subunit (RelA). The p50/p65 heterodimer is present in the cytoplasm in inactive form bound to its inhibitor, IdeltaB. Upon stimulation of cells with stimuli like LPS, Interleukin-1, IkB gets phosphorylated and degraded by proteosomes releasing the activated NFkappaB which goes to the nucleus inducing gene expression. NFkappaB was originally identified as transcriptional activator for kappa light chain gene in B cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: avian reticuloendotheliosis viral (v-rel) oncogene homolog A; NF-kappa-B p65delta3; NF-kappa-B transcription factor p65; NF-kappaB transcription factor p65 subunit; nf?b; nuclear factor kappa B subunit p65; Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3; p65 NF kappaB; p65 NF-kappa B; p65 NFkB; Putative transcription factor p65 homolog; Transcription factor p65; v-rel avian reticuloendotheliosis viral oncogene homolog A; v-rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 (p65)); v-rel reticuloendotheliosis viral oncogene homolog A; v-rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappa light polypeptide gene enhancer in B-cells 3, p65; XRel1
Gene Aliases: BOS_24973; I79_021812; NFkB; NFKB3; p65; rel1; RELA; rela-a; XELAEV_18022301mg; Xrel1; xrela