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Invitrogen
NGAL Monoclonal Antibody (29)
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FIGURE: 1 / 6
NGAL Antibody (ABS 043-29-02) in ICC/IF
Immunofluorescence analysis of NGAL was performed using 70% confluent log phase RAW 264.7 treated with Thapsigargin (250 nm, 48hours). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with NGAL Monoclonal Antibody (29) (Product # ABS 043-29-02) at 5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
ABS 043-29-02
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Rat
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
29
Immunogen
Recombinant mouse NGAL adsorbed onto aluminum hydroxide gel.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.92 mg/mL
Amount
368 µg
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
15mM sodium azide
Storage conditions
4°C, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
ABS 043-29-02 binds free NGAL in mouse serum.
This antibody will not cross react with human, pig, dog, or rat.
ABS 043-29-02 may be used in Western blot and ELISA applications.
NOTE: Concentration is lot-dependent and can vary from 0.85-1.15 mg/mL
Target Information
NGAL (Neutrophil gelatinase-associated lipocalin, lipocalin 2, siderocalin and neutrophil lipocalin) is a 25 kDa glycosylated single chain monomer and member of the lipocalin family of proteins that bind and transport small lipophilic molecules. NGAL is released by activated neutrophils, can form dimers and small amounts of higher oligomers, and complexes with matrix metalloproteinase 9 (MMP-9, gelatinase B). Low level expression of NGAL in a variety of epithelia may be increased in inflammation or cancers. Expression of NGAL in the kidney is dramatically increased by ischemic or nephrotoxic kidney injury.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: HGNC:6526; Lipocalin2; neu-related lipocalin; oncogene 24p3; OTTHUMP00000022240; p25; RP23-161B9.11; secreted inducible protein 24; siderocalin LCN2; SV-40-induced 24P3 protein; unnamed protein product; uterocalin
Gene Aliases: 24p3; NRL; Sip24
UniProt ID: (Mouse) P11672
Entrez Gene ID: (Mouse) 16819
acute-phase response
response to oxidative stress
short-term memory
long-term memory
response to xenobiotic stimulus
response to virus
response to bacterium
response to herbicide
response to toxic substance
response to blue light
response to fructose
response to iron(II) ion
response to mycotoxin
positive regulation of endothelial cell migration
positive regulation of gene expression
siderophore transport
positive regulation of cell projection organization
response to nutrient levels
cellular response to nutrient levels
response to lipopolysaccharide
cellular response to increased oxygen levels
defense response to bacterium
positive regulation of apoptotic process
positive regulation of neuron apoptotic process
innate immune response
cellular response to hydrogen peroxide
cellular response to lipopolysaccharide
cellular response to interleukin-1
cellular response to interleukin-6
cellular response to tumor necrosis factor
cellular response to hypoxia
cellular response to X-ray
extrinsic apoptotic signaling pathway in absence of ligand
positive regulation of hippocampal neuron apoptotic process
negative regulation of hippocampal neuron apoptotic process
positive regulation of cold-induced thermogenesis
positive regulation of reactive oxygen species biosynthetic process
response to kainic acid
positive regulation of iron ion import across plasma membrane
cellular response to beta-amyloid
positive regulation of endothelial tube morphogenesis
cellular response to nerve growth factor stimulus
positive regulation of reactive oxygen species metabolic process
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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