|ELISA (ELISA)||Assay dependent|
|Immunohistochemistry (IHC)||Assay dependent|
|Western Blot (WB)||Assay dependent|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Recombinant human NGAL in monomer and dimer forms|
|Storage buffer||PBS, pH 7.4|
|Contains||15mM sodium azide|
|Storage conditions||4° C, store in dark|
HYB 211-01-02 is specific for human NGAL, native and denatured forms. No cross-reaction to NGAL from other species has been detected.
HYB 211-01-02 has been successfully used in Western blotting, ELISA and Immunohistochemistry procedures. HYB 211-01-02 reacts strongly with NGAL. Strong reaction is seen when tested in sandwich ELISA in combination with a polyclonal antibody against the antigen. In immunohistochemical staining of paraformaldehyde fixed neutrophils HYB 211-01-02 recognizes NGAL. In western blotting HYB 211-01-02 reacts with NGAL in both reduced as well as unreduced form.
The HYB 211-01-02 immunogen is recombinant human NGAL in monomer and dimer forms absorbed to aluminium hydroxide gel.
The epitope specificity of HYB 211-01-02 differs from that of HYB 211-05-02, and differs from HYB 211-02-02 but overlaps.
NGAL (Neutrophil gelatinase-associated lipocalin, lipocalin 2, siderocalin and neutrophil lipocalin) is a 25 kDa glycosylated single chain monomer and member of the lipocalin family of proteins that bind and transport small lipophilic molecules. NGAL is released by activated neutrophils, can form dimers and small amounts of higher oligomers, and complexes with matrix metalloproteinase 9 (MMP-9, gelatinase B). Low level expression of NGAL in a variety of epithelia may be increased in inflammation or cancers. Expression of NGAL in the kidney is dramatically increased by ischemic or nephrotoxic kidney injury.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 25 kDa alpha-2-microglobulin-related subunit of MMP-9; Lipocalin-2; migration-stimulating factor inhibitor; Neutrophil gelatinase-associated lipocalin; NGAL; Oncogene 24p3; p25; Siderocalin LCN2
Gene Aliases: 24p3; HNL; LCN2; MSFI; NGAL; p25
UniProt ID: (Human) P80188
Entrez Gene ID: (Human) 3934
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