Western blot analysis of Neurotrophin Receptor /NGF-Receptor (p75NGFR) was performed by loading 25 ug of SH-SY5Y (lane 1), SW480 (lane 2) and PC12 (lane 3) cell lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4°C overnight. The membrane was probed with a Neurotrophin Receptor /NGF-Receptor (p75NGFR) monoclonal antibody (Product # MA5-13314) at a dilution of 1:100 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate (Product # 32132). Results show a band at ~66-75 kDa in SH-SY5Y and SW480 cells.
|Tested species reactivity||Cat, Ferret, Human, Non-human primate, Rabbit, Rat|
|Published species reactivity||Rat, Human, Mouse|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||NGFR from A875 melanoma cells|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Western Blot (WB)||1:10-1:200|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-13311 targets Neurotrophin Receptor /NGF-Receptor (p75NGFR) in FACS, WB and ICC/IF applications and shows reactivity with Feline, Ferret, Human, Non-human primate, Rat and Rabbit samples.
The MA5-13311 immunogen is nGFR from A875 melanoma cells.
NGF is important for the development, differentiation, and survival of variety of neuronal and non-neuronal cells. Its action is mediated by binding two distinct receptors, the high affinity p140 and low affinity Nerve Growth Factor (NGF) Receptor (p75NGFR) or Neurotrophin Receptor (p75NTR).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
A phase1 study of stereotactic gene delivery of AAV2-NGF for Alzheimer's disease.
MA5-13311 was used in immunohistochemistry to perform a phase I clinical trial to determine the safety and tolerability of an AAV2-NGF vector intended for therapy of Alzheimer's disease
|Rafii MS,Baumann TL,Bakay RA,Ostrove JM,Siffert J,Fleisher AS,Herzog CD,Barba D,Pay M,Salmon DP,Chu Y,Kordower JH,Bishop K,Keator D,Potkin S,Bartus RT||Alzheimer's and dementia : the journal of the Alzheimer's Association (10:571)||2014|
Rac1b increases with progressive tau pathology within cholinergic nucleus basalis neurons in Alzheimer's disease.
MA5-13311 was used in immunohistochemistry to study the expression of Raclb in cholinergic nucleus basalis neurones and the correlation with tau pathology and Alzheimer's disease progression
|Perez SE,Getova DP,He B,Counts SE,Geula C,Desire L,Coutadeur S,Peillon H,Ginsberg SD,Mufson EJ||The American journal of pathology (180:526)||2012|
Low-affinity nerve growth factor receptor (P75 NGFR) as a marker of perineural invasion in malignant melanomas.
MA5-13311 was used in immunohistochemistry to evaluate low-affinity nerve growth factor receptor as a biomarker of perineural invasion in cutaneous neoplasms
|Chan MM,Tahan SR||Journal of cutaneous pathology (37:336)||2010|
In situ evidence of involvement of Schwann cells in ulcerative colitis: autocrine and paracrine signaling by A disintegrin and metalloprotease-17-mediated tumor necrosis factor alpha production.
MA5-13311 was used in immunohistochemistry to investigate the involvement of S100 protein-positive cells in ulcerative colitis
|Mosnier JF,Jarry A,Camdessanché JP,Antoine JC,Laboisse CL||Human pathology (40:1159)||2009|
Immunophenotypical pleomorphism expression in sudden cardiac death.
MA5-13311 was used in immunohistochemistry to investigate the expression of some histopathological and immunohistochemical markers in sudden cardiac death
|Ceau¿u M,Curc¿ C,Ardeleanu C,Dermengiu D||Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie (49:315)||2008|
Immunohistochemical expression patterns of neural and neuroendocrine markers, the neural growth factor receptors and the beta-tubulin II and IV isotypes in human thymus.
MA5-13311 was used in immunohistochemistry to study the expression of the neural growth factor receptors and beta-tubulin II and IV isotypes in human thymus
|Bai M,Papoudou-Bai A,Karatzias G,Doukas M,Goussia A,Stefanaki K,Rontogianni D,Dalavanga Y,Agnantis NJ,Kanavaros P||Anticancer research (28:295)||2008|
Immunohistochemical localization of epithelial rests of Malassez in human periodontal membrane.
MA5-13311 was used in immunohistochemistry to identify epithelial rests of Malassez in human periodontal membrane
|Becktor KB,Nolting D,Becktor JP,Kjaer I||European journal of orthodontics (29:350)||2007|
The activated nerve growth factor receptor p-TrkA is selectively expressed in advanced-stage ovarian carcinoma.
MA5-13311 was used in immunohistochemistry to study the expression of the activated nerve growth factor receptor p-TrkA in advanced-stage ovarian carcinoma
|Ødegaard E,Staff AC,Abeler VM,Kopolovic J,Onsrud M,Lazarovici P,Davidson B||Human pathology (38:140)||2007|
Growth of benign and malignant schwannoma xenografts in severe combined immunodeficiency mice.
MA5-13311 was used in immunohistochemistry to evaluate a human schwannoma xenograft murine model
|Chang LS,Jacob A,Abraham J,Lorenz M,Rock J,Akhmametyeva EM,Mihai G,Schmalbrock P,Chaudhury AR,Lopez R,Yamate J,John MR,Wickert H,Neff BA,Dodson E,Welling DB||The Laryngoscope (116:2018)||2006|
Origin and ontogeny of mammalian ovarian neurons.
MA5-13311 was used in immunohistochemistry to examine the neurogenesis in mammalian ovaries
|Dees WL,Hiney JK,McArthur NH,Johnson GA,Dissen GA,Ojeda SR||Endocrinology (147:3789)||2006|
A histological and anatomical profile of pacinian corpuscles from Dupuytren's contracture and the expression of nerve growth factor receptor.
MA5-13311 was used in immunohistochemistry to study the role of nerve growth factor in the growth of pacinian corpuscles in Dupuytren's disease
|Ehrmantant WR,Graham WP,Towfighi J,Mackay DR,Ehrlich HP||Plastic and reconstructive surgery (114:721)||2004|
Differential expression of the neurotrophin receptors p75NTR, TrkA, TrkB and TrkC in human peripheral blood mononuclear cells.
MA5-13311 was used in flow cytometry to study the expression of neurotrophin receptors in human peripheral blood mononuclear cells
|Nassenstein C,Möhring UH,Luttmann W,Virchow JC,Braun A||Experimental and toxicologic pathology : official journal of the Gesellschaft fur Toxikologische Pathologie (57 Suppl 2:55)||2006|
Crucial role of FOXP3 in the development and function of human CD25+CD4+ regulatory T cells.
MA5-13311 was used in flow cytometry to investigate the role of FOXP3 during specific T cell development
|Yagi H,Nomura T,Nakamura K,Yamazaki S,Kitawaki T,Hori S,Maeda M,Onodera M,Uchiyama T,Fujii S,Sakaguchi S||International immunology (16:1643)||2004|
The p75 neurotrophin receptor is widely expressed in conventional papillary thyroid carcinoma.
MA5-13311 was used in immunoprecipitation to study the expression of the p75 neurotrophin receptor in conventional papillary thyroid carcinoma
|Rocha AS,Risberg B,Magalhães J,Trovisco V,de Castro IV,Lazarovici P,Soares P,Davidson B,Sobrinho-Simões M||Human pathology (37:562)||2006|
The neurotrophins nerve growth factor, brain-derived neurotrophic factor, neurotrophin-3, and neurotrophin-4 are survival and activation factors for eosinophils in patients with allergic bronchial asthma.
MA5-13311 was used in immunocytochemistry to study the role of neurotrophins as survival and activation factors for eosinophils in allergic bronchial asthma
|Nassenstein C,Braun A,Erpenbeck VJ,Lommatzsch M,Schmidt S,Krug N,Luttmann W,Renz H,Virchow JC||The Journal of experimental medicine (198:455)||2003|