|Tested species reactivity||Rabbit, Rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Fusion protein representing the C-terminal 131 amino acids of rabbit NHE3.|
|Storage buffer||tris glycine with 0.15M NaCl|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||1:10-1:500|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:500|
|Western Blot (WB)||0.5 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody has 83% sequence homology with mouse.
Does not react with human samples.
Suggested positive control: rat kidney membrane.
Na+/H+ exchangers-1–8 (also designated NHE-1–8 or Na+/H+ antiporters) are integral membrane proteins that are expressed in most mammalian tissues, where they regulate intracellular pH and cell volume. NHEs mediate the transport of hydrogen (H+) ions out o
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