|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Mouse|
|Host / Isotype||Mouse / IgG|
|Immunogen||Fusion protein containing the C-terminal 89 residues of human NHE8.|
|Storage buffer||tris glycine with 0.15M NaCl|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
Do not boil samples prior to Western Blot.
Suggested positive control: antigen standard for SLC9A8 (transient overexpression lysate), HeLa whole cell lysate..
NHE (Na+/H+ Exchanger) family members are integral membrane proteins which have been identified to play a key role in catalyzing the extrusion of intracellular proton (H+) ions in exchange for extracellular sodium (Na+) ions for regulation of cellular pH as well as transepithelial ion/water transport. NHE8 is a relatively new member of Na+/H+ exchanger family and is a ubiquitously expressed protein with highest levels detected in epithelial cells of the intestine and the kidney as well as in skeletal muscles. Interestingly, the intestinal/renal expression levels of NHE8 are significantly higher than that of NHE2/NHE3 at a young age, suggesting that NHE8 as an important player for sodium absorption during early development. Localized mainly in Golgi apparatus membranes as multi-pass membrane protein, NHE8 is implicated in regulation of pH to eliminate acids generated by active metabolism or to counter adverse environmental conditions. NHE8 is a major proton extruding system driven by the inward sodium ion chemical gradient and has also been proposed to play role in signaling mechanisms also.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Glucocorticoids Reduce Renal NHE8 Expression.
MA1-46357 was used in western blot to study the reduction in renal NHE8 expression in response to glucocorticoids
|Joseph C,Gattineni J,Dwarakanath V,Baum M||Physiological reports (1:null)||2013|