|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Synthetic peptide corresponding to a region of human NIFK between residues 100-200.|
|Storage buffer||PBS with 0.2% gelatin|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||0.5-3 µg/ml|
Suggested positive control: antigen standard for MKI67IP (transient overexpression lysate), HeLa or Ramos.
NIFK is a nucleolar protein that interacts with Ki67, a protein strongly associated with, and a specific marker of, cell proliferation. The marsupial counterpart of human NIFK was recently identified and suggests that NIFK may be involved in the organization of higher order chromatin structure. NIFK was found to interact with the forkhead associated domain of Ki67 in mitotic cells and the interaction is thought to be phosphorylation-dependent.
hNIFK; Ki67ip; MKI67 (FHA domain) interacting nucleolar phosphoprotein; nucleolar phosphoprotein Nopp34; nucleolar protein interacting with the FHA domain of pKi-67
MKI67IP; NIFK; NOPP34