|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A peptide corresponding to amino acids 931 to 947 of human NIK.|
|Contains||0.02% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Western Blot (WB)||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
A suggested positive control is 293 cell lysate.
PA5-19871 can be used with blocking peptide PEP-0007.
Nuclear factor kappa B (NF-kappa-B) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-kappa-B mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNF-a, LPS and mitogens. A serine/threonine protein kinase which mediates NF-kappa-B activation by IL-1, TNF-a and CD95 was identified recently and designated NIK (for NF-kappa-B inducing kinase). NIK is an activator of Ikappa-B kinase alpha and beta (IKK-a and IKK-b). Therefore, NIK is a key molecule in the NF-kappa-B signaling pathway leading to the induction of a variety of gene expression in response to proinflammatory cytokines and bacteria products.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.