NeoBiotechnologies

NKX3.1 Monoclonal Antibody (NKX3.1, 3350)

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NKX3.1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry (Paraffin) analysis performed in formalin fixed, paraffin-embedded human prostate carcinoma using NKX3.1 Monoclonal Antibody (Product # 4824-MSM10-P1).

NKX3.1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry (Paraffin) analysis performed in formalin fixed, paraffin-embedded human prostate carcinoma using NKX3.1 Monoclonal Antibody (Product # 4824-MSM10-P1).

NKX3.1 Antibody in Peptide array (ARRAY) — Analysis of Protein Array containing more than 19,000 full-length human proteins NKX3.1 Monoclonal Antibody (Product # 4824-MSM10-P1). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SDís) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDís) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

NKX3.1 Antibody — SDS-PAGE Analyis Purified NKX3.1 Monoclonal Antibody (Product # 4824-MSM10-P1). Confirmation of Purity and Integrity of Antibody.

Product Details

4824-MSM10-P1

Applications	Tested Dilution	Publications
Immunohistochemistry (Paraffin) (IHC (P))	1-2 µg/mL	-
Peptide Array (Array)	Assay-dependent	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Mouse / IgG2b, kappa
Class	Monoclonal
Type	Antibody
Clone	NKX3.1, 3350
Immunogen	Recombinant fragment (around aa 92-224) of human NKX3.1 protein View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	200 µg/mL
Purification	Protein A
Storage buffer	PBS, pH 7.4, with 0.05% BSA
Contains	0.05% sodium azide
Storage conditions	4° C

Product Specific Information

Immunohistochemistry (PFA fixed): incubate antibody for 30 min RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 degrees C followed by cooling at RT for 20 minutes.

Target Information

The homeodomain-containing transcription factor NKX3-1 is a putative prostate tumor suppressor that is expressed in a largely prostate-specific and androgen-regulated manner. Loss of NKX3-1 protein expression is a common finding in human prostate carcinomas and prostatic intraepithelial neoplasia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Homeobox protein NK-3 homolog A; Homeobox protein Nkx-3.1; NK homeobox, family 3, A; NK3 transcription factor homolog A; NK3 transcription factor related, locus 1; NKX3-1 antibody; prostate-specific ag

Gene Aliases: BAPX2; NKX3; NKX3-1; NKX3.1; NKX3A

UniProt ID: (Human) Q99801

Entrez Gene ID: (Human) 4824

Molecular Function: homeodomain transcription factor

metanephros development somitogenesis positive regulation of protein phosphorylation transcription, DNA-templated regulation of transcription, DNA-templated activation of cysteine-type endopeptidase activity involved in apoptotic process multicellular organism development salivary gland development heart development positive regulation of cell proliferation negative regulation of cell proliferation male gonad development positive regulation of gene expression negative regulation of gene expression positive regulation of cell death positive regulation of phosphatidylinositol 3-kinase signaling androgen receptor signaling pathway activation of protein kinase activity response to testosterone cellular response to drug dorsal aorta development positive regulation of cysteine-type endopeptidase activity involved in apoptotic process protein kinase B signaling negative regulation of insulin-like growth factor receptor signaling pathway negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated negative regulation of mitotic cell cycle positive regulation of mitotic cell cycle positive regulation of transcription from RNA polymerase II promoter branching morphogenesis of an epithelial tube negative regulation of epithelial cell proliferation positive regulation of cell division pharyngeal system development branching involved in prostate gland morphogenesis epithelial cell proliferation involved in salivary gland morphogenesis negative regulation of epithelial cell proliferation involved in prostate gland development cellular response to interleukin-1 cellular response to tumor necrosis factor cellular response to steroid hormone stimulus cellular response to hypoxia mitotic cell cycle arrest negative regulation of estrogen receptor binding positive regulation of androgen secretion positive regulation of response to DNA damage stimulus positive regulation of apoptotic signaling pathway positive regulation of intrinsic apoptotic signaling pathway

Process(es)

transcription regulatory region sequence-specific DNA binding transcription factor activity, RNA polymerase II core promoter sequence-specific core promoter binding transcription factor activity, sequence-specific DNA binding androgen receptor activity protein binding transcription factor binding cysteine-type endopeptidase activator activity involved in apoptotic process estrogen receptor activity protein kinase activator activity estrogen receptor binding histone deacetylase binding sequence-specific DNA binding protein self-association transcription regulatory region DNA binding MADS box domain binding