|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||synthetic peptide PRLPQTPKQPQKRS conjugated to BSA, corresponding to amino acids 70-83 of Human NKX3A, isoform v1.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 50% ammonium sulfate|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:200-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-28069 detects NKX3A from human, mouse, and rat samples.
PA1-28069 has been successfully used in immunohistochemistry, and Western blot applications. Detects a band of approximately 21.6 -26.3 kDa.
The PA1-28069 immunogen is synthetic peptide : PRLPQTPKQPQKRS conjugated to BSA, corresponding to amino acids 70-83 of Human NKX3A, isoform v1.
Reconstituted antibody may be stored at 4°C for up to one month. Addition of a preservative (15 mM sodium azide) may be necessary. For extended storage, add an equal volume of high purity glycerol, to a final concentration of 50% and BSA to a final concentration of 1% and store at -20°C.
The homeodomain-containing transcription factor NKX3-1 is a putative prostate tumor suppressor that is expressed in a largely prostate-specific and androgen-regulated manner. Loss of NKX3-1 protein expression is a common finding in human prostate carcinomas and prostatic intraepithelial neoplasia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.