Western blot analysis of NLRP3 in human transfected cell lysate (A), mouse transfected cell lysate (B) and recombinant human NLRP3 (Pyrin domain) immunogen (C), probed with 5 ug/ml (tranfected) or 0.5 ug/ml (recombinant) NLRP3 antibody (Product # MA5-16275) followed by detection using an HRP-conjugated secondary antibody.
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Mouse / IgG2b, kappa|
|Immunogen||Recombinant mNLRP3 pyrin domain (AA 6-89).|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||2-5 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This gene encodes a pyrin-like protein containing a pyrin domain, a nucleotide-binding site domain, and a leucine-rich repeat motif. This protein interacts with the apoptosis-associated speck-like protein PYCARD/ASC, which contains a caspase recruitment domain, and is a member of the NALP3 inflammasome complex. This complex functions as an upstream activator of NF-kappaB signaling, and it plays a role in the regulation of inflammation, the immune response, and apoptosis. Mutations in this gene are associated with familial cold autoinflammatory syndrome syndrome, and neonatal-onset multisystem inflammatory disease . Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. Alternative 5' UTR structures are suggested by available data; however, insufficient evidence is available to determine if all of the represented 5' UTR splice patterns are biologically valid.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.