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Immunohistochemistry analysis of NMDA1 receptor showing staining in the cytoplasm and membrane of paraffin-embedded human brain tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10 mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a NMDA1 receptor Mouse monoclonal antibody (320500) diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
|Tested species reactivity||Human , Rat , Mouse , Primate|
|Published species reactivity||Rat , Hamster , Human , Mouse|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Fusion protein containing sequence from the intracellular loop between transmembrane regions III and IV of NMDAR1.|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||0.1-1.0 ug/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:50|
|Immunoprecipitation (IP)||1-3 µg/ml|
|Western Blot (WB)||1-3 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
NMDA receptor subtype of glutamate-gated ion channels possesses high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. Plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. It mediates neuronal functions in glutamate neurotransmission. Is involved in the cell surface targeting of NMDA receptors. The ion channels activated by glutamate are divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by kainate and a-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as kainate/AMPA receptors (K/AMPAR). NMDA receptors are among the most studied receptors in neuroscience because they are involved in neuronal cell development and plasticity, a cellular correlate for learning.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
LY395756, an mGluR2 agonist and mGluR3 antagonist, enhances NMDA receptor expression and function in the normal adult rat prefrontal cortex, but fails to improve working memory and reverse MK801-induced working memory impairment.
32-0500 was used in western blot to study how activation of mGluR2/3 affects NMDAR function.
|Li ML,Yang SS,Xing B,Ferguson BR,Gulchina Y,Li YC,Li F,Hu XQ,Gao WJ||Experimental neurology (273:190)||2015|
Mutant disrupted-in-schizophrenia 1 in astrocytes: focus on glutamate metabolism.
32-0500 was used in western blot to characterize the mutant disrupted-in-schizophrenia 1 protein in astrocytes
|Abazyan S,Yang EJ,Abazyan B,Xia M,Yang C,Rojas C,Slusher B,Sattler R,Pletnikov M||Journal of neuroscience research (92:1659)||2014|
Redistribution of ionotropic glutamate receptors detected by laser microdissection of the rat dentate gyrus 48 h following LTP induction in vivo.
32-0500 was used in western blot to study the effect of LTP induction on the distribution of ionotropic glutamate receptors in vivo
|Kennard JT,Guévremont D,Mason-Parker SE,Abraham WC,Williams JM||PloS one (9:null)||2014|
Increased expression, but not postsynaptic localisation, of ionotropic glutamate receptors during the late-phase of long-term potentiation in the dentate gyrus in vivo.
||Kennard JT,Guévremont D,Mason-Parker SE,Abraham WC,Williams JM||Neuropharmacology (56:66)||2009|
Differential trafficking of AMPA and NMDA receptors during long-term potentiation in awake adult animals.
||Williams JM,Guévremont D,Mason-Parker SE,Luxmanan C,Tate WP,Abraham WC||The Journal of neuroscience : the official journal of the Society for Neuroscience (27:14171)||2007|
Long-term regulation of N-methyl-D-aspartate receptor subunits and associated synaptic proteins following hippocampal synaptic plasticity.
||Williams JM,Guévremont D,Kennard JT,Mason-Parker SE,Tate WP,Abraham WC||Neuroscience (118:1003)||2003|
Regional, cellular, and ultrastructural distribution of N-methyl-D-aspartate receptor subunit 1 in monkey hippocampus.
||Siegel SJ,Brose N,Janssen WG,Gasic GP,Jahn R,Heinemann SF,Morrison JH||Proceedings of the National Academy of Sciences of the United States of America (91:564)||1994|
GluN2B-Containing NMDA Receptors Regulate AMPA Receptor Traffic through Anchoring of the Synaptic Proteasome.
32-0500 was used in immunocytochemistry to assess the role of the GluN2B subunit during development and in synaptic plasticity
|Ferreira JS,Schmidt J,Rio P,Águas R,Rooyakkers A,Li KW,Smit AB,Craig AM,Carvalho AL||The Journal of neuroscience : the official journal of the Society for Neuroscience (35:8462)||2015|
Shank expression is sufficient to induce functional dendritic spine synapses in aspiny neurons.
||Roussignol G,Ango F,Romorini S,Tu JC,Sala C,Worley PF,Bockaert J,Fagni L||The Journal of neuroscience : the official journal of the Society for Neuroscience (25:3560)||2005|
NMDA receptors mediate synaptic competition in culture.
||She K,Craig AM||PloS one (6:null)||2011|
Androgenic regulation of tubulin gene expression in axotomized hamster facial motoneurons.
||Jones KJ,Oblinger MM||The Journal of neuroscience : the official journal of the Society for Neuroscience (14:3620)||1994|
Nmdar, Rgsc174, GluRdelta1, GluRzeta1, NMDA1, NMDAR1, M100174, GluN1, MRD8, NR1, RP11-350O14.1, NMD-R1
N-methyl-D-aspartate glutamate receptor, N-methyl-D-aspartate receptor subunit NR1, NMD-R1, NMDA R1 receptor C1 cassette, glutamate [NMDA] receptor subunit zeta-1, glutamate receptor ionotropic, NMDA 1, neurotransmitter receptor, N-methyl-D-aspartate receptor channel, subunit zeta-1, glutamate [NMDA] receptor subunit zeta 1, GLURZ1, GRIN1, Glutamate [NMDA] receptor subunit zeta 1 precursor, NMDZ1, NMZ1, NR1