Immunofluorescence analysis of NMDA Receptor 1 was done on 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with NMDA Receptor 1 Rabbit Polyclonal Antibody (PA3102) at 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d is a merged image showing membranous localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Rat, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues (195)N Y E N L D Q L S Y D N K R G P(210) C of Exon 5 of rat NMDA Receptor Type 1.|
|Storage buffer||whole serum|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay dependent|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:600|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA3-102 detects N-methyl-D-aspartate (NMDA) receptor type 1.
PA3-102 has been used successfully in Western blot, ELISA, immunoprecipitation, immunohistochemistry, and immunocytochemistry procedures. In Western blot analysis of rat brain synaptic membranes this antibody detects a ~120 kDa protein representing NMDA receptor type 1.
The PA3-102 immunogen is a synthetic peptide corresponding to residues (195)N Y E N L D Q L S Y D N K R G P(210) C of Exon 5 of rat NMDA Receptor Type 1.
NMDA receptor subtype of glutamate-gated ion channels possesses high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. Plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. It mediates neuronal functions in glutamate neurotransmission. Is involved in the cell surface targeting of NMDA receptors. The ion channels activated by glutamate are divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by kainate and a-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as kainate/AMPA receptors (K/AMPAR). NMDA receptors are among the most studied receptors in neuroscience because they are involved in neuronal cell development and plasticity, a cellular correlate for learning.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Low levels of methyl ß-cyclodextrin disrupt GluA1-dependent synaptic potentiation but not synaptic depression.
PA3-102 was used in western blot to study the effects of methyl beta-cyclodextrin on GluA1-dependent synaptic potentiation
|Choi TY,Jung S,Nah J,Ko HY,Jo SH,Chung G,Park K,Jung YK,Choi SY||Journal of neurochemistry (132:276)||2015|
Hippocampal long-term potentiation is disrupted during expression and extinction but is restored after reinstatement of morphine place preference.
PA3-102 was used in western blot to study the reinstatement of morphine conditioned place preference and the role of hippocampal synaptic plasticity and NMDAR expression
|Portugal GS,Al-Hasani R,Fakira AK,Gonzalez-Romero JL,Melyan Z,McCall JG,Bruchas MR,Morón JA||The Journal of neuroscience : the official journal of the Society for Neuroscience (34:527)||2014|
Hijacking the neuronal NMDAR signaling circuit to promote tumor growth and invasion.
PA3-102 was used in flow cytometry and immunohistochemistry to study the role of NMDAR signaling in tumorigenesis using a murine model
|Li L,Hanahan D||Cell (153:86)||2013|
Impacts of forebrain neuronal glycine transporter 1 disruption in the senescent brain: evidence for age-dependent phenotypes in Pavlovian learning.
PA3-102 was used in immunohistochemistry to investigate the role of forebrain neuronal glycine transporter 1 in cognitive functions
|Dubroqua S,Singer P,Boison D,Feldon J,Möhler H,Yee BK||Behavioral neuroscience (124:839)||2010|