|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Rat, Mouse|
|Host / Isotype||Mouse|
|Immunogen||Fusion protein containing amino acids 1-564 of the NR1 subunit of rat GRIN1|
|Storage buffer||tissue culture supernatant, pH 7.4|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunoprecipitation (IP)||3 µg/200µg lysate|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
In Western blot, this antibody detects a single ~120 kDa protein representing NR1 in rat brain homogenate. It shows no cross-reactivity with any of the NR2 subunits.
Reconstitute with 50 ul of PBS.
The protein encoded by this gene is a critical subunit of N-methyl-D-aspartate receptors, members of the glutamate receptor channel superfamily which are heteromeric protein complexes with multiple subunits arranged to form a ligand-gated ion channel. These subunits play a key role in the plasticity of synapses, which is believed to underlie memory and learning. Cell-specific factors are thought to control expression of different isoforms, possibly contributing to the functional diversity of the subunits. Alternatively spliced transcript variants have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
The human immunodeficiency virus coat protein gp120 promotes forward trafficking and surface clustering of NMDA receptors in membrane microdomains.
OMA1-04010 was used in immunocytochemistry to investigate the effect of HIV gp120 on NMDA receptor transport in neurons
|Xu H,Bae M,Tovar-y-Romo LB,Patel N,Bandaru VV,Pomerantz D,Steiner JP,Haughey NJ||The Journal of neuroscience : the official journal of the Society for Neuroscience (31:17074)||2011|
Reduced cortical BDNF expression and aberrant memory in Carf knock-out mice.
OMA1-04010 was used in western blot to identify the role of the CaRF transcription factor in neural circuits underlying learning and memory
|McDowell KA,Hutchinson AN,Wong-Goodrich SJ,Presby MM,Su D,Rodriguiz RM,Law KC,Williams CL,Wetsel WC,West AE||The Journal of neuroscience : the official journal of the Society for Neuroscience (30:7453)||2010|
Prion protein expression modulates neuronal copper content.
OMA1-04010 was used in western blot to investigate the relationship between prion protein expression and neuronal copper content
|Brown DR||Journal of neurochemistry (87:377)||2003|