|Immunohistochemistry (Frozen) (IHC (F))||1:1000 to 1:2000|
|Western Blot (WB)||1:2,000|
|Tested Species reactivity||Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to amino acid residues specific to the NR1 subunit of rat GRIN1 , C2 splice variant insert conjugated to KLH|
|Storage buffer||5mM Ammonium bicarbonate|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
This antibody is predicted to react with human based on 100% sequence homology.
In Western blot, this antibody detects an ~100 kDa protein representing the NR1 C2 splice variant from rat brain homogenate. It shows no cross-reactivity with the NR1 N1, C1, or C2 splice variants.
Reconstitute with 50 ul of PBS.
NMDA receptor subtype of glutamate-gated ion channels possesses high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. Plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. It mediates neuronal functions in glutamate neurotransmission. Is involved in the cell surface targeting of NMDA receptors. The ion channels activated by glutamate are divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by kainate and a-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as kainate/AMPA receptors (K/AMPAR). NMDA receptors are among the most studied receptors in neuroscience because they are involved in neuronal cell development and plasticity, a cellular correlate for learning.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: GluN1; Glutamate [NMDA] receptor subunit zeta-1; Glutamate receptor ionotropic, NMDA 1; N-methyl-D-aspartate glutamate receptor; N-Methyl-D-Aspartate Receptor; N-methyl-D-aspartate receptor subunit NR1; neurotransmitter receptor; NMD-R1; NMDA R1 receptor C1 cassette; NR1C2; pGluN1
Gene Aliases: GluN1; GluRdelta1; Glurz1; GluRzeta1; Grin1; M100174; NMD-R1; Nmdar; NMDAR1; NR1; Rgsc174
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.