|Tested species reactivity||Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to amino acid residues specific to the NR1 subunit of rat GRIN1 , N1 splice variant insert conjugated to KLH|
|Storage buffer||5mM Ammonium bicarbonate|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||1:1000 to 1:2000|
|Western Blot (WB)||1:2,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with canine, chicken, human, non-human primate and Xenopus based on 100% sequence homology.
In Western blot, this antibody detects an ~100 kDa protein representing the NR1 N1 splice variant from rat brain homogenate. It shows no cross-reactivity with the NR1 C1, C2, or C2 splice variants.
Reconstitute with 50 ul of PBS. After reconstitution the antibody should be aliquotted and stored at -20°C.
NMDA receptor subtype of glutamate-gated ion channels possesses high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. Plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. It mediates neuronal functions in glutamate neurotransmission. Is involved in the cell surface targeting of NMDA receptors. The ion channels activated by glutamate are divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by kainate and a-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as kainate/AMPA receptors (K/AMPAR). NMDA receptors are among the most studied receptors in neuroscience because they are involved in neuronal cell development and plasticity, a cellular correlate for learning.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.