Immunohistochemistry analysis of NMDA Receptor 2B showing staining in the cytoplasm and membrane of paraffin-embedded human breast tissue (right) compared with a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a NMDA Receptor 2B monoclonal antibody (Product # MA1-2014) diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Rat, Mouse, Human, Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Recombinant protein: NR2B subunit of amino acid residues 934-1457.|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||1 ug/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:100|
|Western Blot (WB)||2 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-2014 detects the N-methyl-D-aspartate (NMDA) receptor type 2B in human and mouse samples.
MA1-2014 has been used successfully in Western blot, IHC (P) and immunocytochemistry procedures. In Western blot analysis of mouse brain tissue this antibody detects a ~166 kDa protein representing NMDA receptor type 2B.
The MA1-2014 immunogen is a recombinant protein composed of amino acid residues 934-1457 of the rat NMDA receptor type 2B.
The ion channels activated by glutamate that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer and quote;s, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). The NMDA receptor is also one of the principal molecular targets for alcohol in the CNS (Lovinger et al., 1989; Alvestad et al., 2003; Snell et al., 1996). The rat NMDAR1 (NR1) was the first subunit of the NMDAR to be clonedand it can form NMDA activated channels when expressed in Xenopus oocytes but the currents in such channels are much smaller than those seen in situ. Channels with more physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits. Overexpression of the NR2B-subunit of the NMDA receptor has been associated with increases in learning and memory while aged, memory impaired animals have deficiencies in NR2B expression (Clayton et al., 2002a; Clayton et al., 2002b). The NMDAR is also potentiated by protein phosphorylation (Lu et al., 1999).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Neurodegeneration in Amygdala Precedes Hippocampus in the APPswe/ PS1dE9 Mouse Model of Alzheimer's Disease.
MA1-2014 was used in western blot to elucidate the APPswe/PS1dE9 mouse model of Alzheimer's disease that shows neurodegeneration in amygdala precedes hippocampus
|Lin TW,Liu YF,Shih YH,Chen SJ,Huang TY,Chang CY,Lien CH,Yu L,Chen SH,Kuo YM||Current Alzheimer research (12:951)||2015|
Environmental enrichment mitigates the sex-specific effects of gestational inflammation on social engagement and the hypothalamic pituitary adrenal axis-feedback system.
MA1-2014 was used in western blot to study the effect of environment enrichment on maternal immune activation
|Connors EJ,Shaik AN,Migliore MM,Kentner AC||Brain, behavior, and immunity (42:178)||2014|
Disulfide-containing high mobility group box-1 promotes N-methyl-D-aspartate receptor function and excitotoxicity by activating Toll-like receptor 4-dependent signaling in hippocampal neurons.
MA1-2014 was used in western blot to study the role of hippocampal neuronal TLR4 signaling activation in the mechanism by which HMGB1 drives NMDAR hyperexcitability
|Balosso S,Liu J,Bianchi ME,Vezzani A||Antioxidants and redox signaling (21:1726)||2014|
The influence of neuropathology on brain inflammation in human and experimental temporal lobe epilepsy.
MA1-2014 was used in western blot to study whether neuropathological changes contribute to temporal lobe epilepsy brain inflammation
|Aalbers MW,Rijkers K,Majoie HJ,Dings JT,Schijns OE,Schipper S,De Baets MH,Kessels A,Vles JS,Hoogland G||Journal of neuroimmunology (271:36)||2014|
Mitigation of augmented extrasynaptic NMDAR signaling and apoptosis in cortico-striatal co-cultures from Huntington's disease mice.
MA1-2014 was used in western blot to study changes in NMDAR signaling and apoptosis in cortico-striatal co-cultures from Huntington's disease mice
|Milnerwood AJ,Kaufman AM,Sepers MD,Gladding CM,Zhang L,Wang L,Fan J,Coquinco A,Qiao JY,Lee H,Wang YT,Cynader M,Raymond LA||Neurobiology of disease (48:40)||2012|
Age and meloxicam attenuate the ischemia/reperfusion-induced down-regulation in the NMDA receptor genes.
MA1-2014 was used in western blot to study NMDA receptor gene responses to ischemia
|Montori S,Dos-Anjos S,Martínez-Villayandre B,Regueiro-Purriños MM,Gonzalo-Orden JM,Ruano D,Fernández-López A||Neurochemistry international (56:878)||2010|
Characterization of NMDA receptor subunit-specific antibodies: distribution of NR2A and NR2B receptor subunits in rat brain and ontogenic profile in the cerebellum.
MA1-2014 was used in western blot to investigate the localization of NMDA receptor subunits in the rat brain and the corresponding expression changes during development using novel subunit-specific antibodies
|Wang YH,Bosy TZ,Yasuda RP,Grayson DR,Vicini S,Pizzorusso T,Wolfe BB||Journal of neurochemistry (65:176)||1995|
TRPV1 acts as a synaptic protein and regulates vesicle recycling.
MA1-2014 was used in immunocytochemistry to study the role TRPV1 as a synaptic protein regulating vesicle recycling
|Goswami C,Rademacher N,Smalla KH,Kalscheuer V,Ropers HH,Gundelfinger ED,Hucho T||Journal of cell science (123:2045)||2010|
Nonspecific interaction of prefibrillar amyloid aggregates with glutamatergic receptors results in Ca2+ increase in primary neuronal cells.
MA1-2014 was used in immunocytochemistry to study the Ca(2+) increase in neuronal cells induced by the non-specific interaction of pre-fibrillar amyloid aggregates with glutaminergic receptors
|Pellistri F,Bucciantini M,Relini A,Nosi D,Gliozzi A,Robello M,Stefani M||The Journal of biological chemistry (283:29950)||2008|