Immunofluorescence analysis of NMDA RECEPTOR 2B was performed using 70% confluent log phase RSC96 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with NMDAR2B Rabbit Polyclonal Antibody (718600) at 2µg/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing membranous localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Rabbit, Rat|
|Published species reactivity||Dog, Rat, Non-human primate, Mouse, Human, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Fusion protein including a 251 amino acid sequence from the N-terminal portion of the rat NMDAR 2B protein.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/ml|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Blocking Assay (BLOCK)||See 1 publications below|
|Miscellaneous PubMed (MISC)||See 2 publications below|
|Western Blot (WB)||See 5 publications below|
|Immunohistochemistry (IHC)||See 1 publications below|
|Immunohistochemistry - Free Floating (IHC (Free))||See 1 publications below|
|Immunofluorescence (IF)||See 1 publications below|
|Immunoprecipitation (IP)||See 1 publications below|
N-methyl-D-aspartate (NMDA) receptors are a class of ionotropic glutamate receptors. NMDA receptor channel has been shown to be involved in long-term potentiation, an activity-dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. NMDA receptor channels are heteromers composed of three different subunits: NR1 (GRIN1), NR2 (GRIN2A, GRIN2B, GRIN2C, or GRIN2D) and NR3 (GRIN3A or GRIN3B). The NR2 subunit acts as the agonist binding site for glutamate. This receptor is the predominant excitatory neurotransmitter receptor in the mammalian brain.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Stereotactic injection of cerebrospinal fluid from anti-NMDA receptor encephalitis into rat dentate gyrus impairs NMDA receptor function.
71-8600 was used in blocking or activating experiment to determine impairment of NMDA receptor function by sterotactic injection of cerebrospinal fluid from anti-NMDA receptor encephalitis into rat dentate gyrus
|Würdemann T,Kersten M,Tokay T,Guli X,Kober M,Rohde M,Porath K,Sellmann T,Bien CG,Köhling R,Kirschstein T||Brain research (1633:10)||2016|
Defective Age-Dependent Metaplasticity in a Mouse Model of Alzheimer's Disease.
71-8600 was used in western blot to test if LTP/LTD changes in Alzheimer's disease mouse models indicate defects in metaplasticity processes
|Megill A,Tran T,Eldred K,Lee NJ,Wong PC,Hoe HS,Kirkwood A,Lee HK||The Journal of neuroscience : the official journal of the Society for Neuroscience (35:11346)||2015|
N-methyl-D-aspartate receptor subunit phenotypes of vagal afferent neurons in nodose ganglia of the rat.
71-8600 was used in immunohistochemistry (frozen) to examine NMDA receptor subunit immunoreactivity in vagal afferent neurons.
|Czaja K,Ritter RC,Burns GA||The Journal of comparative neurology (496:877)||2006|
A tetra(ethylene glycol) derivative of benzothiazole aniline enhances Ras-mediated spinogenesis.
71-8600 was used in western blot to study the effect of a novel amyloid-binding small molecule on Ras-mediated spinogenesis
|Megill A,Lee T,DiBattista AM,Song JM,Spitzer MH,Rubinshtein M,Habib LK,Capule CC,Mayer M,Turner RS,Kirkwood A,Yang J,Pak DT,Lee HK,Hoe HS||The Journal of neuroscience : the official journal of the Society for Neuroscience (33:9306)||2013|
ALLN rescues an in vitro excitatory synaptic transmission deficit in Lis1 mutant mice.
71-8600 was used in western blot to test the therapeutic potential of ALLN, a calpain inhibitor, on postnatal lissencephalic cells.
|Sebe JY,Bershteyn M,Hirotsune S,Wynshaw-Boris A,Baraban SC||Journal of neurophysiology (109:429)||2013|
Polyglutamine-modulated striatal calpain activity in YAC transgenic huntington disease mouse model: impact on NMDA receptor function and toxicity.
71-8600 was used in western blot to report that the cumulative effect of increasing huntingtin polyglutamine length enhancement of medium-sized spiny neuron sensitivity to excitotoxicity
|Cowan CM,Fan MM,Fan J,Shehadeh J,Zhang LY,Graham RK,Hayden MR,Raymond LA||The Journal of neuroscience : the official journal of the Society for Neuroscience (28:12725)||2008|
|Rat||Not Cited||Differential trafficking of AMPA and NMDA receptors during long-term potentiation in awake adult animals.||Williams JM,Guévremont D,Mason-Parker SE,Luxmanan C,Tate WP,Abraham WC||The Journal of neuroscience : the official journal of the Society for Neuroscience (27:14171)||2007|
|Mouse||Not Cited||Long-term regulation of N-methyl-D-aspartate receptor subunits and associated synaptic proteins following hippocampal synaptic plasticity.||Williams JM,Guévremont D,Kennard JT,Mason-Parker SE,Tate WP,Abraham WC||Neuroscience (118:1003)||2003|
Phosphorylation of N-methyl-D-aspartic acid receptor-associated neuronal nitric oxide synthase depends on estrogens and modulates hypothalamic nitric oxide production during the ovarian cycle.
71-8600 was used in immunohistochemistry to investigate the relationship between neuronal NO synthase and estrogen.
|Parkash J,d'Anglemont de Tassigny X,Bellefontaine N,Campagne C,Mazure D,Buée-Scherrer V,Prevot V||Endocrinology (151:2723)||2010|
Distribution of glutamate receptor subunits in experimentally induced cortical malformations.
71-8600 was used in immunohistochemistry - free floating to examine the regional expression of four NMDA receptor subunits and two major AMPA- and kainate-receptor complexes in adult rats after neonatal freeze lesions.
|Hagemann G,Kluska MM,Redecker C,Luhmann HJ,Witte OW||Neuroscience (117:991)||2003|
|Dual palmitoylation of PSD-95 mediates its vesiculotubular sorting, postsynaptic targeting, and ion channel clustering.||El-Husseini AE,Craven SE,Chetkovich DM,Firestein BL,Schnell E,Aoki C,Bredt DS||The Journal of cell biology (148:159)||2000|
|Human||Not Cited||PSD-95 assembles a ternary complex with the N-methyl-D-aspartic acid receptor and a bivalent neuronal NO synthase PDZ domain.||Christopherson KS,Hillier BJ,Lim WA,Bredt DS||The Journal of biological chemistry (274:27467)||1999|