Invitrogen

NMDAR1 Monoclonal Antibody (54.1)

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

28 Published Figures

48 References

View all (36) NMDAR1 antibodies

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FIGURE: 1 / 34

NMDAR1 Antibody (32-0500) in WB

Western blot analysis of NMDA 1 Receptor was performed by loading 30 µg of Mouse Brain (lane1) and Rat Brain (lane2) tissue lysate using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX), XCell SureLock™ Electrophoresis System (Product # EI0002), Novex® Sharp Pre-Stained Protein Standard (LC5800), and Pierce™ Power Blotter System (22834). Proteins were transferred to a nitrocellulose membrane and blocked with 5 % skim milk at 4°C overnight. NMDA 1 Receptor was detected at ~103 kDa using NMDA 1 Receptor Mouse Monoclonal Antibody (Prod... View More

Product Image Gallery

NMDAR1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of MAP2 (green) and NMDAR1 (red) on rat primary Hippocampal neurons (E18) (Product # A15587) cultured for 28 days in the B-27 Plus Neuronal Culture System (Product # A3653401). At day 28 the cells were fixed with 4% paraformaldehyde for 15 min, permeabilized with 0.1% triton x-100 for 30min, and blocked with 1% BSA for 30 min at room temperature. Cells were stained with anti-NMDAR1 antibody (Product # 32-0500) at a dilution of 1:100, and anti-MAP2 (Product # PA5-17646) at a dilution of 1:250, in 1% BSA staining buffer, overnight at 4C, and then incubated with Alexa Fluor 488 conjugated donkey anti-rabbit (Product # A-21206) and Alexa Fluor 594 donkey anti-mouse (Product # A-21203) antibodies at a dilution of 1:1000 for 30 min. at room temp. Wash 3 times with DPBS. Stain with DAPI for nucleus.

NMDAR1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of NMDA1 receptor showing staining in the cytoplasm and membrane of paraffin-embedded human brain tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10 mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a NMDA1 receptor Mouse monoclonal antibody (Product # 32-0500) diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

NMDAR1 Antibody in Immunoprecipitation (IP) — Immunoprecipitation of NMDA 1 Receptor was performed with 5 µg of the NMDA 1 Receptor Mouse Monoclonal Antibody (Product # 32-0500) on tissue extract from Rat Brain using the Dynabeads® Protein A Immunoprecipitation Kit (10006D). Normal Mouse IgG was used as a negative IP control. Subsequently, western blot analysis was performed using Novex® NuPAGE® 10 % Bis-Tris gel (Product # NP0301BOX), XCell SureLock™ Electrophoresis System (Product # EI0002), Novex® Sharp Pre-Stained Protein Standard (LC5800). Proteins were transferred using iBlot® 2 Dry Blotting System (IB21001) to a nitrocellulose membrane and blocked with 5% skim milk for 1 hour at room temperature on a rocking platform. NMDA 1 Receptor was detected at ~ 103 kDa using NMDA 1 Receptor Mouse Monoclonal Antibody (Product # 32-0500) at 1-3 µg/mL in 5 % skim milk at 4°C overnight on a rocking platform. Goat Anti-Mouse - HRP Secondary Antibody (Product # 62-6520) at 1:4000 dilution was used and chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106). Lane 1 represents 10 % of the total tissue extract (input), Lane 2 is the IP performed with Mouse IgG and Lane 3 represents IP performed with NMDA 1 Receptor Mouse Monoclonal Antibody (Product # 32-0500).

NMDAR1 Antibody in Immunohistochemistry (Frozen) (IHC (F)) — 7 Fig GABAARalpha1 and NMADR1 expression by immunofluorescence and DAPI counterstaining in the CA3 field of the hippocampus. (A) Representative immunofluorescence image of the CA3 field. (B) NE|RS group showed significantly increased expression of NMADR1 (red), whereas expression of GABAARalpha1 (green) was significantly decreased in the RS and NE|RS groups. Error bars indicate SEM. * P < .05, ** P < .005, *** P < .001. An analysis of variance with least significant difference post hoc tests for multiple comparisons was performed to examine immunofluorescence in the CA3 field of the hippocampus. GABAARalpha1 = gamma-aminobutyric acid A receptor alpha 1; NE = noise exposure; NMDAR1 = N -methyl- d -aspartate receptor 1; RS = restraint stress; SEM = standard error of mean

NMDAR1 Antibody in Immunohistochemistry (IHC) — Published figure using NMDAR1 monoclonal antibody (Product # 32-0500) in Immunohistochemistry

NMDAR1 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across Mouse Brain, Mouse Liver owing to their inherent genetic constitution. Relative expression of NMDAR1 was observed in Mouse Brain as compared to Mouse Liver using Anti-NMDAR1 Monoclonal Antibody (54.1) (Product # 32-0500) in Western Blot. {RE}

Product Details

32-0500

Applications

Tested Dilution

Publications

Western Blot (WB)

1-5 µg/mL

View 30 publications 30 publications

Immunohistochemistry (IHC)

View 7 publications 7 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:50

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Immunohistochemistry - Free Floating (IHC (Free))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:100

View 6 publications 6 publications

ELISA (ELISA)

0.1-1 µg/mL

Immunoprecipitation (IP)

1-3 µg/mL

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Published species

Fish, Hamster, Human, Mouse, Rat, Tag

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

54.1

Immunogen

Fusion protein containing sequence from the intracellular loop between transmembrane regions III and IV of NMDAR1.

Conjugate

Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

RRID

AB_2533060

Product Specific Information

This antibody specifically recognizes the ~103 kDa NMDAR1 protein. Cross-reactivity with other NMDA receptor proteins has not been observed.

Target Information

NMDAR1 encodes a protein that is a critical subunit of N-methyl-D-aspartate receptors, members of the glutamate receptor channel superfamily which are heteromeric protein complexes with multiple subunits arranged to form a ligand-gated ion channel. These subunits play a key role in the plasticity of synapses, which is believed to underlie memory and learning. Cell-specific factors are thought to control expression of different isoforms, possibly contributing to the functional diversity of the subunits. Alternatively spliced transcript variants have been described.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Isoflurane impairs GluN2B-containing NMDA receptors trafficking and cognition via decreasing histone acetylation and EphB2 expression in aged hippocampal neurons.

Basic & clinical pharmacology & toxicology

Hao JR,Hu QM,Yang X,Wei P,Wang HY,Sun N,Gao C

32-0500 was used in Western Blotting to highlight the crucial role of histone posttranslational modifications for EphB2-GluN2B signals in isoflurane-associated PND, and modulating HDAC2 might be a new therapeutic strategy for isoflurane-associated PND.

Wed Feb 01 00:00:00 EST 2023

Synaptic Neurofilaments and GluN1-Neurofilament Light Chain Interaction in Experimental Models of α-Synucleinopathies.

Neuro-degenerative diseases

Imarisio A,Ferrari E,Pilotto A,Di Luca M,Padovani A,Gardoni F

Published figure using NMDAR1 monoclonal antibody (Product # 32-0500) in Western Blot

Fri Nov 18 00:00:00 EST 2022

Alteration of Ethanol Reward by Prior Mephedrone Exposure: The Role of Age and Matrix Metalloproteinase-9 (MMP-9).

International journal of molecular sciences

Grochecki P,Smaga I,Marszalek-Grabska M,Lopatynska-Mazurek M,Slowik T,Gibula-Tarlowska E,Kedzierska E,Listos J,Filip M,Kotlinska JH

32-0500 was used in Western Blotting to suggest that prior mephedrone exposure differentially alters ethanol reward in adolescent and adult rats.

Mon Feb 14 00:00:00 EST 2022

Molecular Mechanisms Underlying the Retrieval and Extinction of Morphine Withdrawal-Associated Memories in the Basolateral Amygdala and Dentate Gyrus.

Biomedicines

Franco-García A,Fernández-Gómez FJ,Gómez-Murcia V,Hidalgo JM,Milanés MV,Núñez C

32-0500 was used in Immunohistochemistry to provide evidence of differential expression and activity of synaptic molecules during the retrieval and extinction of aversive memories of opiate withdrawal.

Wed Mar 02 00:00:00 EST 2022

Evidence of Tinnitus Development Due to Stress: An Experimental Study in Rats.

The Laryngoscope

Kim MJ,Park SY,Park JM,Yu HJ,Park I,Park SN

Published figure using NMDAR1 monoclonal antibody (Product # 32-0500) in Immunohistochemistry (Frozen)

Fri Oct 01 00:00:00 EDT 2021

Evidence of Tinnitus Development Due to Stress: An Experimental Study in Rats.

The Laryngoscope

Kim MJ,Park SY,Park JM,Yu HJ,Park I,Park SN

Published figure using NMDAR1 monoclonal antibody (Product # 32-0500) in Immunohistochemistry (Frozen)

Fri Oct 01 00:00:00 EDT 2021

Effects of Mephedrone and Amphetamine Exposure during Adolescence on Spatial Memory in Adulthood: Behavioral and Neurochemical Analysis.

International journal of molecular sciences

Grochecki P,Smaga I,Lopatynska-Mazurek M,Gibula-Tarlowska E,Kedzierska E,Listos J,Talarek S,Marszalek-Grabska M,Hubalewska-Mazgaj M,Korga-Plewko A,Dudka J,Marzec Z,Filip M,Kotlinska JH

Published figure using NMDAR1 monoclonal antibody (Product # 32-0500) in Western Blot

Fri Jan 08 00:00:00 EST 2021

Effects of Mephedrone and Amphetamine Exposure during Adolescence on Spatial Memory in Adulthood: Behavioral and Neurochemical Analysis.

International journal of molecular sciences

Grochecki P,Smaga I,Lopatynska-Mazurek M,Gibula-Tarlowska E,Kedzierska E,Listos J,Talarek S,Marszalek-Grabska M,Hubalewska-Mazgaj M,Korga-Plewko A,Dudka J,Marzec Z,Filip M,Kotlinska JH

Published figure using NMDAR1 monoclonal antibody (Product # 32-0500) in Western Blot

Fri Jan 08 00:00:00 EST 2021

Synaptic protein interaction networks encode experience by assuming stimulus-specific and brain-region-specific states.

Cell reports

Lautz JD,Tsegay KB,Zhu Z,Gniffke EP,Welsh JP,Smith SEP

32-0500 was used in Immunoprecipitation to conclude that although the PIN of the glutamatergic post-synapse is expressed widely throughout the brain, its activity-dependent dynamics show remarkable stimulus-specific and brain-region-specific diversity.

Tue Nov 30 00:00:00 EST 2021

Cocaine Self-Administration and Abstinence Modulate NMDA Receptor Subunits and Active Zone Proteins in the Rat Nucleus Accumbens.

Molecules (Basel, Switzerland)

Smaga I,Wydra K,Frankowska M,Fumagalli F,Sanak M,Filip M

Published figure using NMDAR1 monoclonal antibody (Product # 32-0500) in Western Blot

Fri Jul 31 00:00:00 EDT 2020

Cocaine Self-Administration and Abstinence Modulate NMDA Receptor Subunits and Active Zone Proteins in the Rat Nucleus Accumbens.

Molecules (Basel, Switzerland)

Smaga I,Wydra K,Frankowska M,Fumagalli F,Sanak M,Filip M

Published figure using NMDAR1 monoclonal antibody (Product # 32-0500) in Western Blot

Fri Jul 31 00:00:00 EDT 2020

Glutamate Receptor Trafficking and Protein Synthesis Mediate the Facilitation of LTP by Secreted Amyloid Precursor Protein-Alpha.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Mockett BG,Guévremont D,Elder MK,Parfitt KD,Peppercorn K,Morrissey J,Singh A,Hintz TJ,Kochen L,Tom Dieck S,Schuman E,Tate WP,Williams JM,Abraham WC

Published figure using NMDAR1 monoclonal antibody (Product # 32-0500) in Western Blot

Wed Apr 24 00:00:00 EDT 2019

Amyloid-β Oligomers Regulate ADAM10 Synaptic Localization Through Aberrant Plasticity Phenomena.

Molecular neurobiology

Marcello E,Musardo S,Vandermeulen L,Pelucchi S,Gardoni F,Santo N,Antonucci F,Di Luca M

Published figure using NMDAR1 monoclonal antibody (Product # 32-0500) in Western Blot

Tue Oct 01 00:00:00 EDT 2019

PSD-95 deficiency disrupts PFC-associated function and behavior during neurodevelopment.

Scientific reports

Coley AA,Gao WJ

32-0500 was used in Western Blotting to highlight the importance of PSD-95 during neurodevelopment in the mPFC and its potential link in the pathogenesis associated with schizophrenia and/or autism.

Mon Jul 01 00:00:00 EDT 2019

Juvenile treatment with mGluR2/3 agonist prevents schizophrenia-like phenotypes in adult by acting through GSK3β.

Neuropharmacology

Xing B,Han G,Wang MJ,Snyder MA,Gao WJ

32-0500 was used in Western Blotting to provide a mechanism for mGluR2/3 agonists against NMDAR hypofunction.

Sun Jul 15 00:00:00 EDT 2018

The histone demethylase KDM6B in the medial prefrontal cortex epigenetically regulates cocaine reward memory.

Neuropharmacology

Zhang YX,Akumuo RC,España RA,Yan CX,Gao WJ,Li YC

32-0500 was used in Western Blotting to suggest that KDM6B plays an essential role in cocaine-associated memory, which mainly acts through enhancing cocaine-induced synaptic plasticity in the mPFC.

Mon Oct 01 00:00:00 EDT 2018

Epigenetic mechanisms underlying NMDA receptor hypofunction in the prefrontal cortex of juvenile animals in the MAM model for schizophrenia.

Journal of neurochemistry

Gulchina Y,Xu SJ,Snyder MA,Elefant F,Gao WJ

Published figure using NMDAR1 monoclonal antibody (Product # 32-0500) in Western Blot

Wed Nov 01 00:00:00 EDT 2017

Hierarchical organization and genetically separable subfamilies of PSD95 postsynaptic supercomplexes.

Journal of neurochemistry

Frank RAW,Zhu F,Komiyama NH,Grant SGN

32-0500 was used in Immunohistochemistry to gain insight into the mechanisms that govern the constituents of postsynaptic supercomplexes and the diversity of synapse types.

Tue Aug 01 00:00:00 EDT 2017

Juvenile treatment with a novel mGluR2 agonist/mGluR3 antagonist compound, LY395756, reverses learning deficits and cognitive flexibility impairments in adults in a neurodevelopmental model of schizophrenia.

Neurobiology of learning and memory

Li ML,Gulchina Y,Monaco SA,Xing B,Ferguson BR,Li YC,Li F,Hu XQ,Gao WJ

Published figure using NMDAR1 monoclonal antibody (Product # 32-0500) in Western Blot

Sat Apr 01 00:00:00 EDT 2017

NMDA receptors participate in the progression of diabetic kidney disease by decreasing Cdc42-GTP activation in podocytes.

The Journal of pathology

Shen J,Wang R,He Z,Huang H,He X,Zhou J,Yan Y,Shen S,Shao X,Shen X,Weng C,Lin W,Chen J

Published figure using NMDAR1 monoclonal antibody (Product # 32-0500) in Western Blot

Sat Oct 01 00:00:00 EDT 2016

NMDA receptors participate in the progression of diabetic kidney disease by decreasing Cdc42-GTP activation in podocytes.

The Journal of pathology

Shen J,Wang R,He Z,Huang H,He X,Zhou J,Yan Y,Shen S,Shao X,Shen X,Weng C,Lin W,Chen J

Published figure using NMDAR1 monoclonal antibody (Product # 32-0500) in Western Blot

Sat Oct 01 00:00:00 EDT 2016

NMDA receptors are selectively partitioned into complexes and supercomplexes during synapse maturation.

Nature communications

Frank RA,Komiyama NH,Ryan TJ,Zhu F,O'Dell TJ,Grant SG

Published figure using NMDAR1 monoclonal antibody (Product # 32-0500) in Western Blot

Wed Apr 27 00:00:00 EDT 2016

Pentobarbital modifies the lipid raft-protein interaction: A first clue about the anesthesia mechanism on NMDA and GABA_A receptors.

Biochimica et biophysica acta

Sierra-Valdez FJ,Ruiz-Suárez JC,Delint-Ramirez I

320500 was used in western blot to determine the in vivo effects of pentobarbital on brain plasma membranes and lipid rafts

Tue Nov 01 00:00:00 EDT 2016

GluN2B-Containing NMDA Receptors Regulate AMPA Receptor Traffic through Anchoring of the Synaptic Proteasome.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Ferreira JS,Schmidt J,Rio P,Águas R,Rooyakkers A,Li KW,Smit AB,Craig AM,Carvalho AL

32-0500 was used in immunocytochemistry to assess the role of the GluN2B subunit during development and in synaptic plasticity

Wed Jun 03 00:00:00 EDT 2015

LY395756, an mGluR2 agonist and mGluR3 antagonist, enhances NMDA receptor expression and function in the normal adult rat prefrontal cortex, but fails to improve working memory and reverse MK801-induced working memory impairment.

Experimental neurology

Li ML,Yang SS,Xing B,Ferguson BR,Gulchina Y,Li YC,Li F,Hu XQ,Gao WJ

32-0500 was used in western blot to study how activation of mGluR2/3 affects NMDAR function.

Sun Nov 01 00:00:00 EDT 2015

Mutant disrupted-in-schizophrenia 1 in astrocytes: focus on glutamate metabolism.

Journal of neuroscience research

Abazyan S,Yang EJ,Abazyan B,Xia M,Yang C,Rojas C,Slusher B,Sattler R,Pletnikov M

Published figure using NMDAR1 monoclonal antibody (Product # 32-0500) in Western Blot

Mon Dec 01 00:00:00 EST 2014

Redistribution of ionotropic glutamate receptors detected by laser microdissection of the rat dentate gyrus 48 h following LTP induction in vivo.

PloS one

Kennard JT,Guévremont D,Mason-Parker SE,Abraham WC,Williams JM

32-0500 was used in western blot to study the effect of LTP induction on the distribution of ionotropic glutamate receptors in vivo

Fri Jan 30 00:00:00 EST 2015

Metabotropic glutamate receptor 5 mediates phosphorylation of vascular endothelial cadherin and nuclear localization of β-catenin in response to homocysteine.

Vascular pharmacology

Beard RS,Reynolds JJ,Bearden SE

Published figure using NMDAR1 monoclonal antibody (Product # 32-0500) in Immunohistochemistry

Tue Jul 17 00:00:00 EDT 2012

Metabotropic glutamate receptor 5 mediates phosphorylation of vascular endothelial cadherin and nuclear localization of β-catenin in response to homocysteine.

Vascular pharmacology

Beard RS,Reynolds JJ,Bearden SE

Published figure using NMDAR1 monoclonal antibody (Product # 32-0500) in Immunohistochemistry

Tue Jul 17 00:00:00 EDT 2012

Metabotropic glutamate receptor 5 mediates phosphorylation of vascular endothelial cadherin and nuclear localization of β-catenin in response to homocysteine.

Vascular pharmacology

Beard RS,Reynolds JJ,Bearden SE

Published figure using NMDAR1 monoclonal antibody (Product # 32-0500) in Immunohistochemistry

Tue Jul 17 00:00:00 EDT 2012

Metabotropic glutamate receptor 5 mediates phosphorylation of vascular endothelial cadherin and nuclear localization of β-catenin in response to homocysteine.

Vascular pharmacology

Beard RS,Reynolds JJ,Bearden SE

Published figure using NMDAR1 monoclonal antibody (Product # 32-0500) in Immunohistochemistry

Tue Jul 17 00:00:00 EDT 2012

Metabotropic glutamate receptor 5 mediates phosphorylation of vascular endothelial cadherin and nuclear localization of β-catenin in response to homocysteine.

Vascular pharmacology

Beard RS,Reynolds JJ,Bearden SE

Published figure using NMDAR1 monoclonal antibody (Product # 32-0500) in Immunohistochemistry

Tue Jul 17 00:00:00 EDT 2012

Metabotropic glutamate receptor 5 mediates phosphorylation of vascular endothelial cadherin and nuclear localization of β-catenin in response to homocysteine.

Vascular pharmacology

Beard RS,Reynolds JJ,Bearden SE

Published figure using NMDAR1 monoclonal antibody (Product # 32-0500) in Immunohistochemistry

Tue Jul 17 00:00:00 EDT 2012

Glutamate binding to the GluN2B subunit controls surface trafficking of N-methyl-D-aspartate (NMDA) receptors.

The Journal of biological chemistry

She K,Ferreira JS,Carvalho AL,Craig AM

32-0500 was used in immunocytochemistry to study the control of NMDA receptor surface trafficking by the binding of glutamate to GluN2B subunits.

Fri Aug 10 00:00:00 EDT 2012

Long-term consequences of a prolonged febrile seizure in a dual pathology model.

Neurobiology of disease

Gibbs S,Chattopadhyaya B,Desgent S,Awad PN,Clerk-Lamalice O,Levesque M,Vianna RM,Rébillard RM,Delsemme AA,Hébert D,Tremblay L,Lepage M,Descarries L,Di Cristo G,Carmant L

32-0500 was used in western blot to identify the long term electrophysiological, anatomical and molecular changes in a rat model of temporal lobe epilepsy.

Mon Aug 01 00:00:00 EDT 2011

Stargazin and AMPA receptor membrane expression is increased in the somatosensory cortex of Genetic Absence Epilepsy Rats from Strasbourg.

Neurobiology of disease

Kennard JT,Barmanray R,Sampurno S,Ozturk E,Reid CA,Paradiso L,D'Abaco GM,Kaye AH,Foote SJ,O'Brien TJ,Powell KL

32-0500 was used in western blot to measure the developmental expression of stargazin and AMPA receptor trafficking in the Genetic Absence Epilepsy Rats from Strasbourg model.

Fri Apr 01 00:00:00 EDT 2011

NMDA receptors mediate synaptic competition in culture.

PloS one

She K,Craig AM

Tue Feb 28 00:00:00 EST 2012

In vivo composition of NMDA receptor signaling complexes differs between membrane subdomains and is modulated by PSD-95 and PSD-93.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Delint-Ramirez I,Fernández E,Bayés A,Kicsi E,Komiyama NH,Grant SG

Published figure using NMDAR1 monoclonal antibody (Product # 32-0500) in Western Blot

Wed Jun 16 00:00:00 EDT 2010

N-methyl-D-aspartate, hyperpolarization-activated cation current (Ih) and gamma-aminobutyric acid conductances govern the risk of epileptogenesis following febrile seizures in rat hippocampus.

The European journal of neuroscience

Ouardouz M,Lema P,Awad PN,Di Cristo G,Carmant L

Published figure using NMDAR1 monoclonal antibody (Product # 32-0500) in Western Blot

Thu Apr 01 00:00:00 EDT 2010

N-methyl-D-aspartate, hyperpolarization-activated cation current (Ih) and gamma-aminobutyric acid conductances govern the risk of epileptogenesis following febrile seizures in rat hippocampus.

The European journal of neuroscience

Ouardouz M,Lema P,Awad PN,Di Cristo G,Carmant L

Published figure using NMDAR1 monoclonal antibody (Product # 32-0500) in Western Blot

Thu Apr 01 00:00:00 EDT 2010

The importance of N-methyl-D-aspartate (NMDA) receptors in subtraction of electrosensory reafference in the dorsal nucleus of skates.

The Journal of experimental biology

Zhang Z,Bodznick D

Sun Aug 01 00:00:00 EDT 2010

The importance of N-methyl-D-aspartate (NMDA) receptors in subtraction of electrosensory reafference in the dorsal nucleus of skates.

The Journal of experimental biology

Zhang Z,Bodznick D

Sun Aug 01 00:00:00 EDT 2010

The importance of N-methyl-D-aspartate (NMDA) receptors in subtraction of electrosensory reafference in the dorsal nucleus of skates.

The Journal of experimental biology

Zhang Z,Bodznick D

Sun Aug 01 00:00:00 EDT 2010

Increased expression, but not postsynaptic localisation, of ionotropic glutamate receptors during the late-phase of long-term potentiation in the dentate gyrus in vivo.

Neuropharmacology

Kennard JT,Guévremont D,Mason-Parker SE,Abraham WC,Williams JM

Thu Jan 01 00:00:00 EST 2009

Differential trafficking of AMPA and NMDA receptors during long-term potentiation in awake adult animals.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Williams JM,Guévremont D,Mason-Parker SE,Luxmanan C,Tate WP,Abraham WC

Wed Dec 19 00:00:00 EST 2007

Expression of AMPA and NMDA receptor subunits in the cervical spinal cord of wobbler mice.

BMC neuroscience

Bigini P,Gardoni F,Barbera S,Cagnotto A,Fumagalli E,Longhi A,Corsi MM,Di Luca M,Mennini T

Published figure using NMDAR1 monoclonal antibody (Product # 32-0500) in Immunohistochemistry

Thu Oct 26 00:00:00 EDT 2006

Expression of AMPA and NMDA receptor subunits in the cervical spinal cord of wobbler mice.

BMC neuroscience

Bigini P,Gardoni F,Barbera S,Cagnotto A,Fumagalli E,Longhi A,Corsi MM,Di Luca M,Mennini T

Published figure using NMDAR1 monoclonal antibody (Product # 32-0500) in Immunohistochemistry

Thu Oct 26 00:00:00 EDT 2006

Shank expression is sufficient to induce functional dendritic spine synapses in aspiny neurons.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Roussignol G,Ango F,Romorini S,Tu JC,Sala C,Worley PF,Bockaert J,Fagni L

Wed Apr 06 00:00:00 EDT 2005

SNAP-25 reduction in the hippocampus of patients with schizophrenia.

Progress in neuro-psychopharmacology & biological psychiatry

Thompson PM,Egbufoama S,Vawter MP

32-0500 was used in western blot to study synaptosomal associated protein 25 kDa immunoreactivity in the hippocampus of patients with schizophrenia.

Thu May 01 00:00:00 EDT 2003

Long-term regulation of N-methyl-D-aspartate receptor subunits and associated synaptic proteins following hippocampal synaptic plasticity.

Neuroscience

Williams JM,Guévremont D,Kennard JT,Mason-Parker SE,Tate WP,Abraham WC

Fri Jul 11 00:00:00 EDT 2003

Light and electron microscopic distribution of the AMPA receptor subunit, GluR2, in the spinal cord of control and G86R mutant superoxide dismutase transgenic mice.

The Journal of comparative neurology

Morrison BM,Janssen WG,Gordon JW,Morrison JH

Mon Jun 15 00:00:00 EDT 1998

Distribution of glutamate receptor subunit proteins GluR2(4), GluR5/6/7, and NMDAR1 in the canine and primate cerebral cortex: a comparative immunohistochemical analysis.

Brain research

Hof PR,Vissavajjhala P,Rosenthal RE,Fiskum G,Morrison JH

Mon Jun 03 00:00:00 EDT 1996

Distribution of glutamate receptor subunit proteins GluR2(4), GluR5/6/7, and NMDAR1 in the canine and primate cerebral cortex: a comparative immunohistochemical analysis.

Brain research

Hof PR,Vissavajjhala P,Rosenthal RE,Fiskum G,Morrison JH

Mon Jun 03 00:00:00 EDT 1996

Regional, cellular, and ultrastructural distribution of N-methyl-D-aspartate receptor subunit 1 in monkey hippocampus.

Proceedings of the National Academy of Sciences of the United States of America

Siegel SJ,Brose N,Janssen WG,Gasic GP,Jahn R,Heinemann SF,Morrison JH

Tue Jan 18 00:00:00 EST 1994

Androgenic regulation of tubulin gene expression in axotomized hamster facial motoneurons.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Jones KJ,Oblinger MM

Wed Jun 01 00:00:00 EDT 1994

Bioinformatics

Protein Aliases: GluN1; Glun1r; glutamate [NMDA] receptor subunit zeta 1; Glutamate [NMDA] receptor subunit zeta-1; glutamate receptor; Glutamate receptor ionotropic, NMDA 1; glutamate receptor, ionotropic, N-methyl D-aspartate 1; N-methyl-D-aspartate glutamate receptor; N-methyl-D-aspartate receptor channel, subunit zeta-1; N-methyl-D-aspartate receptor subunit NR1; neurotransmitter receptor; NMD-R1; NMDA R1 receptor C1 cassette; NMDA receptor 1; NMDZ1; NMZ1; NR1C1; NR1C2; NR1C2'; NR1N1; pGluN1; RP11-350O14.1

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Gene Aliases: GluN1; GluRdelta1; Glurz1; GluRzeta1; GRIN1; M100174; MRD8; NMD-R1; NMDA1; Nmdar; NMDAR1; NR1; Rgsc174

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UniProt ID: (Human) Q05586, (Rat) P35439, (Mouse) P35438

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Entrez Gene ID: (Human) 2902, (Rat) 24408, (Mouse) 14810

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Molecular Function: transmembrane signal receptor

Function(s)

MAPK cascade conditioned taste aversion suckling behavior response to amphetamine cation transport cellular calcium ion homeostasis chemical synaptic transmission respiratory gaseous exchange long-term memory adult locomotory behavior olfactory learning visual learning propylene metabolic process sensory perception of pain pons maturation cerebral cortex development regulation of ion transmembrane transport social behavior ionotropic glutamate receptor signaling pathway synaptic transmission, glutamatergic regulation of membrane potential positive regulation of apoptotic process response to morphine negative regulation of neuron apoptotic process positive regulation of GTPase activity regulation of respiratory gaseous exchange response to ethanol positive regulation of transcription from RNA polymerase II promoter ephrin receptor signaling pathway regulation of long-term neuronal synaptic plasticity rhythmic process regulation of dendrite morphogenesis regulation of axonogenesis protein tetramerization response to calcium ion regulation of synapse assembly calcium ion homeostasis excitatory postsynaptic potential prepulse inhibition male mating behavior response to fungicide calcium ion transmembrane transport cellular response to manganese ion positive regulation of excitatory postsynaptic potential startle response calcium ion transport learning or memory learning memory associative learning regulation of cell communication positive regulation of cell death response to organic cyclic compound response to amine ion transmembrane transport regulation of neuron apoptotic process regulation of synaptic plasticity regulation of neuronal synaptic plasticity neuromuscular process transport ion transport cation transmembrane transport

Process(es)

NMDA glutamate receptor activity Ras guanyl-nucleotide exchange factor activity extracellular-glutamate-gated ion channel activity calcium channel activity calcium ion binding protein binding calmodulin binding glycine binding glutamate binding enzyme binding voltage-gated cation channel activity glutamate receptor binding neurotransmitter binding protein heterodimerization activity ionotropic glutamate receptor activity receptor binding cation channel activity protein dimerization activity receptor activity ion channel activity

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NMDAR1 Monoclonal Antibody (54.1)

NMDAR1 Antibody (32-0500) in WB

Product Details

32-0500

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunohistochemistry - Free Floating (IHC (Free))

Immunocytochemistry (ICC/IF)

ELISA (ELISA)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

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