|Immunocytochemistry (ICC)||1 ug/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:100|
|Western Blot (WB)||2 ug/ml|
|Western Blot (WB)||See 7 publications below|
|Immunocytochemistry (ICC)||See 2 publications below|
|Tested Species reactivity||Human, Mouse, Rat|
|Published species reactivity||Rat , Mouse , Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Recombinant protein: NR2B subunit of amino acid residues 934-1457.|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
MA1-2014 detects the N-methyl-D-aspartate (NMDA) receptor type 2B in human and mouse samples.
MA1-2014 has been used successfully in Western blot, IHC (P) and immunocytochemistry procedures. In Western blot analysis of mouse brain tissue this antibody detects a ~166 kDa protein representing NMDA receptor type 2B.
The MA1-2014 immunogen is a recombinant protein composed of amino acid residues 934-1457 of the rat NMDA receptor type 2B.
The ion channels activated by glutamate that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer and quote;s, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). The NMDA receptor is also one of the principal molecular targets for alcohol in the CNS (Lovinger et al., 1989; Alvestad et al., 2003; Snell et al., 1996). The rat NMDAR1 (NR1) was the first subunit of the NMDAR to be clonedand it can form NMDA activated channels when expressed in Xenopus oocytes but the currents in such channels are much smaller than those seen in situ. Channels with more physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits. Overexpression of the NR2B-subunit of the NMDA receptor has been associated with increases in learning and memory while aged, memory impaired animals have deficiencies in NR2B expression (Clayton et al., 2002a; Clayton et al., 2002b). The NMDAR is also potentiated by protein phosphorylation (Lu et al., 1999).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: GluN2B; GluRepsilon2; Glutamate [NMDA] receptor subunit epsilon-2; Glutamate receptor ionotropic, NMDA 2B; glutamate receptor subunit epsilon-2; glutamate receptor, ionotropic, N-methyl D-aspartate 2B; glutamate receptor, ionotropic, NMDA2B; N-methyl D-aspartate receptor 2B; N-methyl D-aspartate receptor subtype 2B; N-methyl-D-aspartate receptor subunit 3; N-Methyl-D-Aspartate Receptor Type 2B; NMDAR2B; NR2B; NR3
Gene Aliases: AW490526; EIEE27; GluN2B; GRIN2B; hNR3; MRD6; NMDAR2B; NR2B
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