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| Applications | Tested Dilution | Publications |
|---|---|---|
Western Blot (WB) |
1:10,000-1:25,000 | View 1 publication 1 publication |
Immunocytochemistry (ICC/IF) |
1:2,000-1:20,000 | - |
Immunoprecipitation (IP) |
2-4 µL/lysate | - |
| Product Specifications | |
|---|---|
Species Reactivity |
Yeast |
Published species |
Yeast |
Host/Isotype |
Mouse / IgG3 |
Class |
Monoclonal |
Type |
Antibody |
Clone |
28F2 |
Immunogen |
Yeast nuclear preparation. |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
Conc. Not Determined |
Storage buffer |
tissue culture supernatant |
Contains |
5mM sodium azide |
Storage conditions |
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
RRID |
AB_1077286 |
MA1-10025 detects Yeast NOP-1 from S. cerevisiae samples and does not cross-react with fibrillarin in other species.
For chemiluminescent Western blot using yeast samples, use a dilution of 1:10,000 for cell lysates and 1:25,000 for nuclear fractions. For non-ECL detection methods, use a dilution of 1:1000-1:5000.
Nop1p is the yeast homologue of human fibrillarin, a component of the nucleolar small nuclear ribonucleoprotein (snRNP) particle.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Histone-glutamine methyltransferase; Nop1p; Nucleolar protein 1; rRNA 2'-O-methyltransferase fibrillarin; rRNA methyltransferase NOP1; U3 small nucleolar RNA-associated protein NOP1
Gene Aliases: D2870; LOT3; NOP1; YDL014W
UniProt ID: (Baker's yeast) P15646
Entrez Gene ID: (Baker's yeast) 851548
Molecular Function:
RNA processing factor
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