MA1-91405 detects Notch1 from mouse and human samples.
MA1-91405 has been successfully used in flow cytometry and immunohistochemistry (paraffin tissue) applications.Staining of formalin fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10 - 20 min followed by cooling at room temperature for 20 min. A suggested positive control for this product is pancreas.
The MA1-91405 immunogen is recombinant protein encoding the ligand binding region of human Notch 1.
The notch gene belongs to a family of epidermal growth factor (EGF) like homeotic genes, which encode transmembrane proteins with a variable number of cysteine rich EGF like repeats in the extracellular region. Four notch genes have been described in mammals, which include notch 1. notch 2, notch 3, and notch 4(Int 3), which have been implicated in the differentiation of the nervous system and other structures. The EGF like proteins Delta and Serrate have been identified as ligands of notch 1. Mature notch proteins are heterodimeric receptors derived from the cleavage of notch preproteins into an extracellular subunit (NEC) containing multiple EGF like repeats and a transmembrane subunit including intracellular region (Ntm). Alteration of notch 1 signaling or expression may contribute to tumorigenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: hN1; major type A protein; Motch A; mT14; Neurogenic locus notch homolog protein 1; Notch 1; Notch gene homolog 1; Notch homolog 1, translocation-associated; p300; TAN1; Translocation-associated notch protein TAN-1; transmembrane receptor Notch1
Gene Aliases: 9930111A19Rik; AOS5; AOVD1; hN1; lin-12; Mis6; Motch; N1; NOTCH1; TAN1