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Applications | Tested Dilution | Publications |
---|---|---|
Western Blot (WB) |
- | View 2 publications 2 publications |
Immunohistochemistry (IHC) |
- | View 6 publications 6 publications |
Immunohistochemistry (Paraffin) (IHC (P)) |
1:50 | - |
Immunocytochemistry (ICC/IF) |
- | View 1 publication 1 publication |
Flow Cytometry (Flow) |
Assay-dependent | View 2 publications 2 publications |
Neutralization (Neu) |
- | View 3 publications 3 publications |
Product Specifications | |
---|---|
Species Reactivity |
Human, Mouse |
Published species |
Human, Mouse |
Host/Isotype |
Mouse / IgG2b |
Class |
Monoclonal |
Type |
Antibody |
Clone |
A6 |
Immunogen |
Recombinant protein encoding the ligand binding region of human Notch 1 |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
0.2 mg/mL |
Purification |
Protein A |
Storage buffer |
PBS, pH 7.4, with 0.2% BSA |
Contains |
0.1% sodium azide |
Storage conditions |
4° C, do not freeze |
RRID |
AB_2153330 |
MA1-91405 has been successfully used in flow cytometry and immunohistochemistry (paraffin tissue) applications. Staining of formalin fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10 - 20 min followed by cooling at room temperature for 20 min. A suggested positive control for this product is pancreas.
The notch gene belongs to a family of epidermal growth factor (EGF) like homeotic genes, which encode transmembrane proteins with a variable number of cysteine-rich EGF-like repeats in the extracellular region. Four notch genes have been described in mammals: Notch1, Notch2, Notch3 and Notch4(Int-3), which have been implicated in the differentiation of the nervous system and other structures. The EGF-like proteins Delta and Serrate have been identified as ligands of Notch1. Mature Notch proteins are heterodimeric receptors derived from the cleavage of Notch pre-proteins into an extracellular subunit (NEC) containing multiple EGF-like repeats and a transmembrane subunit including intracellular region (Ntm).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: major type A protein; Motch A; mT14; Neurogenic locus notch homolog protein 1; NEXT; NICD; Notch 1; Notch 1 extracellular truncation; Notch 1 intracellular domain; Notch gene homolog 1; Notch homolog 1, translocation-associated; p300; RP23-306D20.12; RP23-306D20.12-001; Translocation-associated notch protein TAN-1; transmembrane receptor Notch1
Gene Aliases: 9930111A19Rik; AOS5; AOVD1; hN1; lin-12; Mis6; Motch; N1; NOTCH1; TAN1
UniProt ID: (Human) P46531, (Mouse) Q8K428
Entrez Gene ID: (Human) 4851, (Mouse) 18128
Molecular Function:
cell adhesion molecule
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