Immunofluorescent analysis of Notch-1 (green) showing staining in the membrane of NIH-3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Notch-1 monoclonal antibody (Product # MA5-11961) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 100x.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Human, Mouse, Not Applicable|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Recombinant protein encoding the ligand binding region of human Notch-1|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
|Western Blot (WB)||1:100-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-11961 targets Notch-1 in WB, FACS and ICC/IF applications and shows reactivity with Human and mouse samples.
The MA5-11961 immunogen is recombinant protein encoding the ligand binding region of human Notch-1.
The notch gene belongs to a family of epidermal growth factor (EGF) like homeotic genes, which encode transmembrane proteins with a variable number of cysteine-rich EGF-like repeats in the extracellular region. Four notch genes have been described in mammals, which include notch-1. notch-2, notch-3, and notch-4(Int-3), which have been implicated in the differentiation of the nervous system and other structures. The EGF-like proteins delta and Serrate have been identified as ligands of notch-1. Mature notch proteins are heterodimeric receptors derived from the cleavage of notch pre-proteins into an extracellular subunit (NEC) containing multiple EGF-like repeats and a transmembrane subunit including intracellular region (Ntm). Alteration of notch-1 signaling or expression may contribute to tumorigenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Notch1 modulates mesenchymal stem cells mediated regulatory T-cell induction.
MA5-11961 was used in blocking or activating experiment to study the mechanism by which Notch1 regulates regulatory T-cell induction by mesenchymal stem cells
|Del Papa B,Sportoletti P,Cecchini D,Rosati E,Balucani C,Baldoni S,Fettucciari K,Marconi P,Martelli MF,Falzetti F,Di Ianni M||European journal of immunology (43:182)||2013|
Blockage of Notch1 signaling modulates the T-helper (Th)1/Th2 cell balance in chronic hepatitis B patients.
MA5-11961 was used in blocking or activating experiment to investigate the influence of blockage of Notch1 signaling on T-helper cell balance in patients with chronic hepatitis B viral infection
|Pei J,Tang Z,Zang G,Yu Y||Hepatology research : the official journal of the Japan Society of Hepatology (40:799)||2010|
|Not Applicable||Not Cited||
Distinct roles of EGF repeats for the Notch signaling system.
MA5-11961 was used in blocking or activating experiment to report that Notch1 forms homodimers via its EGF motifs
|Sakamoto K,Chao WS,Katsube K,Yamaguchi A||Experimental cell research (302:281)||2005|
Distinct spatial and molecular features of notch pathway assembly in regulatory T cells.
MA5-11961 was used in flow cytometry to study the distinct molecular features of the Notch pathway in regulatory T cells
|Perumalsamy LR,Marcel N,Kulkarni S,Radtke F,Sarin A||Science signaling (5:null)||2012|
Candidate genes on chromosome 9q33-34 involved in the progression of childhood ependymomas.
MA5-11961 was used in flow cytometry to identify genetic alterations in childhood ependymomas
|Puget S,Grill J,Valent A,Bieche I,Dantas-Barbosa C,Kauffmann A,Dessen P,Lacroix L,Geoerger B,Job B,Dirven C,Varlet P,Peyre M,Dirks PB,Sainte-Rose C,Vassal G||Journal of clinical oncology : official journal of the American Society of Clinical Oncology (27:1884)||2009|
Changes in the expression of stem cell markers in oral lichen planus and hyperkeratotic lesions.
MA5-11961 was used in immunohistochemistry to investigate the expression of stem cell markers in normal and diseased buccal mucosa
|Köse O,Lalli A,Kutulola AO,Odell EW,Waseem A||Journal of oral science (49:133)||2007|
Pancreatic regeneration in chronic pancreatitis requires activation of the notch signaling pathway.
MA5-11961 was used in immunohistochemistry to study the role of Notch signaling in pancreatic regeneration in chronic pancreatitis
|Su Y,Büchler P,Gazdhar A,Giese N,Reber HA,Hines OJ,Giese T,Büchler MW,Friess H||Journal of gastrointestinal surgery : official journal of the Society for Surgery of the Alimentary Tract (10:1230)||2006|
Sarcomatoid carcinomas of the lung--are these histogenetically heterogeneous tumors?
MA5-11961 was used in immunohistochemistry to study the histogenetic heterogeneity of sarcomatoid carcinomas of the lung
|Blaukovitsch M,Halbwedl I,Kothmaier H,Gogg-Kammerer M,Popper HH||Virchows Archiv : an international journal of pathology (449:455)||2006|
The Notch signaling pathway is related to neurovascular progression of pancreatic cancer.
MA5-11961 was used in immunohistochemistry to study the role of the Notch pathway in pancreatic cancer angiogenesis and neuroinvasion
|Büchler P,Gazdhar A,Schubert M,Giese N,Reber HA,Hines OJ,Giese T,Ceyhan GO,Müller M,Büchler MW,Friess H||Annals of surgery (242:791)||2005|
Isolation of precursor cells (PCs) from human dental follicle of wisdom teeth.
MA5-11961 was used in immunohistochemistry to isolate and characterize precursor cells from human dental follicle of wisdom teeth
|Morsczeck C,Götz W,Schierholz J,Zeilhofer F,Kühn U,Möhl C,Sippel C,Hoffmann KH||Matrix biology : journal of the International Society for Matrix Biology (24:155)||2005|
Evidence for a role for notch signaling in the cytokine-dependent survival of activated T cells.
MA5-11961 was used in western blot to study the potential role of Notch1 signaling in cytokine-dependent survival of activated T cells
|Bheeshmachar G,Purushotaman D,Sade H,Gunasekharan V,Rangarajan A,Sarin A||Journal of immunology (Baltimore, Md. : 1950) (177:5041)||2006|
NB-3/Notch1 pathway via Deltex1 promotes neural progenitor cell differentiation into oligodendrocytes.
MA5-11961 was used in western blot to study the involvement of NB-3/Notch1 pathway in the development of oligodendrocytes
|Cui XY,Hu QD,Tekaya M,Shimoda Y,Ang BT,Nie DY,Sun L,Hu WP,Karsak M,Duka T,Takeda Y,Ou LY,Dawe GS,Yu FG,Ahmed S,Jin LH,Schachner M,Watanabe K,Arsenijevic Y,Xiao ZC||The Journal of biological chemistry (279:25858)||2004|