This antibody is specific for the Notch1 (neurogenic locus notch homolog protein1, Motch A, mT14, p300) protein. This antibody gives a band of ~65 kDa on Western blot analysis using Notch1-IC OP transfected cells. Western blot analysis using Jurkat cells expresses the target band at ~110-120 kDa, with an additional band occasionally observed at ~220kDa. This antibody does not cross-react with Notch 2,3, and 4 as assessed by Western blot analysis.
The notch gene belongs to a family of epidermal growth factor (EGF) like homeotic genes, which encode transmembrane proteins with a variable number of cysteine-rich EGF-like repeats in the extracellular region. Four notch genes have been described in mammals: Notch1, Notch2, Notch3 and Notch4(Int-3), which have been implicated in the differentiation of the nervous system and other structures. The EGF-like proteins Delta and Serrate have been identified as ligands of Notch1. Mature Notch proteins are heterodimeric receptors derived from the cleavage of Notch pre-proteins into an extracellular subunit (NEC) containing multiple EGF-like repeats and a transmembrane subunit including intracellular region (Ntm).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Drosophila Notch homolog 1 (controlling the the ectodermal and neural cell fate in Drosophila); major type A protein; Motch A; mT14; Neurogenic locus notch homolog protein 1; NEXT; NICD; Notch 1; Notch 1 extracellular truncation; Notch 1 intracellular domain; Notch gene homolog 1; Notch homolog 1, translocation-associated; p300; RP23-306D20.12; RP23-306D20.12-001; Translocation-associated notch protein TAN-1; transmembrane receptor Notch1
Gene Aliases: 9930111A19Rik; AOS5; AOVD1; hN1; lin-12; Mis6; Motch; N1; NOTCH; NOTCH1; TAN1