|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Partially Purified human neutrophil flavocytochrome b (heparin Ultrogellectin affinity purification in Triton X-100)|
|Contains||0.1% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||1 ug/ml|
|Immunofluorescence (IF)||1 ug/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||5 ug/ml|
|Western Blot (WB)||Assay-dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is specific for NOX2/gp91phox (aa 382-389, PKIAVDGP). The immunogen was prepared using partially purified human neutrophil flavocytochrome b (heparin Ultrogellectin affinity purification in Triton X-100).
Western blot results show a band at 65 kDa, which matches the predicted molecular weight. For IHC applications, perform heat-mediated antigen retrieval with citrate buffer, pH 6, prior to staining.
Cytochrome b is composed of cytochrome b alpha and beta chain. It has been proposed as a primary component of the microbicidal oxidase system of phagocytes. CYBB deficiency is one of five described biochemical defects associated with chronic granulomatous disease . In this disorder, there is decreased activity of phagocyte NADPH oxidase; neutrophils are able to phagocytize bacteria but cannot kill them in the phagocytic vacuoles. The cause of the killing defect is an inability to increase the cell's respiration and consequent failure to deliver activated oxygen into the phagocytic vacuole.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.