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|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||5 µg/ml|
|Western Blot (WB)||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 3 publications below|
Suggested positive control: antigen standard for PMAIP1 (transient overexpression lysate), RL-7, Jurkat.
Recently, a BH-3 only member of the Bcl-2 family have been identified in human and mouse and designated as Noxa (for damage) (1). The expression of the Noxa gene involves direct activation of its promoter by p53. Increased expression of Noxa protein occurs in normal thymocytes but not in p53-deficient thymocytes. Noxa cDNA codes for a 103-amino acid protein. The coimmunoprecipitation data suggest that Noxa protein may interact with proteins belonging to the Bcl-2 family, such as, Bcl-XL and Mcl-1. Oda et al (1) have also shown that blocking the endogenous Noxa induction results in the suppression of apoptosis. Treatment of cells with Noxa antisense oligonucleotides blocks radiation-induced apoptosis suggesting that Noxa may represent a mediator of p53-dependent apoptosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Noxa determines localization and stability of MCL-1 and consequently ABT-737 sensitivity in small cell lung cancer.
MA1-41000 was used in western blot to evaluate the effect of Noxa on the localization and stability of MCL-1 in small cell lung cancer
|Nakajima W,Hicks MA,Tanaka N,Krystal GW,Harada H||Cell death & disease (5:null)||2014|
Proteasomal degradation of Mcl-1 by maritoclax induces apoptosis and enhances the efficacy of ABT-737 in melanoma cells.
MA1-41000 was used in western blot to study the effects of the Mcl-1 inhibitor Maritoclax and ABT-737 on melanoma cell survival
|Pandey MK,Gowda K,Doi K,Sharma AK,Wang HG,Amin S||PloS one (8:null)||2013|
BRAF inhibitors suppress apoptosis through off-target inhibition of JNK signaling.
mA1-41000 was used in western blot to study the role of off-target JNK inhibition in the mechanism by which BRAF inhibitors block apoptosis
|Vin H,Ojeda SS,Ching G,Leung ML,Chitsazzadeh V,Dwyer DW,Adelmann CH,Restrepo M,Richards KN,Stewart LR,Du L,Ferguson SB,Chakravarti D,Ehrenreiter K,Baccarini M,Ruggieri R,Curry JL,Kim KB,Ciurea AM,Duvic M,Prieto VG,Ullrich SE,Dalby KN,Flores ER,Tsai KY||eLife (2:null)||2013|
adult T cell leukemia-derived PMA-responsive; APR; immediate-early-response protein APR; NOXA; phorbol-12-myristate-13-acetate-induced protein 1; PMA-induced protein 1; protein Noxa
APR; NOXA; PMAIP1