Invitrogen

NPC1 Recombinant Rabbit Monoclonal Antibody (JB87-33)

Product Image Gallery

NPC1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of Niemann Pick C1 in PC-3M cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Niemann Pick C1 monoclonal antibody (Product # MA5-34694), followed by DAPI (blue).

NPC1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Niemann Pick C1 in paraffin-embedded mouse testis tissue. Samples were incubated with Niemann Pick C1 monoclonal antibody (Product # MA5-34694), and followed by hematoxylin.

NPC1 Antibody in Western Blot (WB) — Western blot analysis of Niemann Pick C1 in SiHa cell lysate. Samples were incubated with Niemann Pick C1 monoclonal antibody (Product # MA5-34694), at a dilution of 1:500.

NPC1 Antibody in Flow Cytometry (Flow) — Flow cytometry of Niemann Pick C1 in SH-SY-5Y cells (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Samples were incubated with Niemann Pick C1 monoclonal antibody (Product # MA5-34694) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti rabbit IgG.

Product Details

MA5-34694

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:1,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:200	-
Immunocytochemistry (ICC/IF)	1:50-1:200	-
Flow Cytometry (Flow)	1:50-1:100	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Rabbit / IgG
Class	Recombinant Monoclonal
Type	Antibody
Clone	JB87-33
Immunogen	Recombinant protein within Human Niemann Pick C1 aa 1,160-1,278 View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Protein A
Storage buffer	TBS, pH 7.4, with 40% glycerol, 0.05% BSA
Contains	0.05% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles, store in dark
RRID	AB_2848602

Product Specific Information

Positive Control: SiHa, A549, HepG2, PC-3M, Rat kidney tissue, human kidney tissue, mouse testis tissue, SH-SY-5Y.

Target Information

Mutations in the Niemann-Pick disease type C1 (NPC1) gene result in a fatal progressive neurodegenerative disorder characterized by an abnormal sequestration of lipids including cholesterol and glycosphingolipids. The NPC1 protein is a large protein that resides in the limiting membrane of endosomes and lysosomes and mediates intracellular cholesterol trafficking via binding of cholesterol to its N-terminal domain. NPC1 transports low-density lipoproteins to late endosomal/lysosomal compartments where they are hydrolyzed and released as free cholesterol. NPC1, in addition to FTO, MC4R, and PTER has recently been shown to be a new risk loci for early-onset and morbid adult obesity in European populations. This anti-NPC1 antibody will not cross-react to NPC2, another gene whose defects also result in Niemann-Pick type C disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?

Let us know so we can reference it here.

Cite this product

Bioinformatics

Protein Aliases: FLJ98532; Niemann-Pick C1 protein; Niemann-Pick disease, type C1; NPC intracellular cholesterol transporter 1; truncated Niemann-Pick C1

Gene Aliases: NPC; NPC1

UniProt ID: (Human) O15118

Entrez Gene ID: (Human) 4864

Function(s)