Invitrogen

NR3C2 Monoclonal Antibody (H10E4C9F)

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NR3C2 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of Mineralocorticoid Receptor using Mineralocorticoid Receptor Monoclonal antibody (H10E4C9F) (Product # MA1-620) shows staining in HEK293 cells. Mineralocorticoid Receptor staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Mineralocorticoid Receptor (Product # MA1-620) at a dilution of 1:20-1:200 over night at 4 °C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35552 for GAR, Product # 35503 for GAM). Images were taken at 60X magnification.

NR3C2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry was performed on cancer biopsies of deparaffinized Human colon carcinoma tissues. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:100 with a mouse monoclonal antibody recognizing Mineralocorticoid Receptor (Product # MA1-620) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

NR3C2 Antibody in Immunohistochemistry (IHC) — Figure 2 GR and MR labeling in the rat amygdala. (A & E) GR-ir neurons are distributed throughout the amygdala predominately labeling the nucleus. (B & F) MR-ir (MA1-620) neurons are distributed throughout the amygdala but immunoreactivity is not as dense as GR-ir. Large proximal dendrites showed immunoreactivity (F inset). (C & G) MR-ir (rMR1-18 1D5) neurons are distributed in amygdala nuclei and cell membrane but do not appear as densely labeled in perikaryon (G inset). (D & H) Control section shows little immunoreactivity of cells in amygdala or surrounding nuclei. Scale bars = 200 um for A-D, 10 um for D-G and 5 um for F & G insets. Amygdala nuclei subdivisions B = basal, Ce = central, Lad = lateral (dorsal division), Lvl = lateral (ventral-lateral), Lvm = lateral (ventral-medial).

NR3C2 Antibody in Western Blot (WB) — Published figure using NR3C2 monoclonal antibody (Product # MA1-620) in Western Blot

NR3C2 Antibody in Immunoprecipitation (IP) — Published figure using NR3C2 monoclonal antibody (Product # MA1-620) in Immunoprecipitation

Product Details

MA1-620

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000	View 7 publications 7 publications
Immunohistochemistry (IHC)	-	View 10 publications 10 publications
Immunohistochemistry (Paraffin) (IHC (P))	1/100	-
Immunocytochemistry (ICC/IF)	Assay-dependent	-
Flow Cytometry (Flow)	1/20	-
Immunoprecipitation (IP)	-	View 1 publication 1 publication
Neutralization (Neu)	-	View 1 publication 1 publication
Inhibition Assays (IA)	Assay-dependent	-

Product Specifications
Species Reactivity	Chicken, Human, Rabbit, Rat
Published species	Chicken, Human, Mouse, Rabbit, Rat, Sheep
Host/Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Clone	H10E4C9F
Immunogen	Aldosterone-3
Conjugate	Unconjugated
Form	Liquid
Concentration	1.4 mg/mL
Purification	Protein A
Storage buffer	PBS
Contains	0.05% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2298880

Product Specific Information

MA1-620 detects mineralocorticoid receptor (MR) in human, rat, rabbit and chicken tissues.

MA1-620 has been successfully used in Western blot, immunocytochemistry and immunohistochemistry procedures. By Western blot, this antibody detects a 116 kDa protein representing MR in rat heart homogenate. Immunohistochemical staining of MR in rabbit atrium with MA1-620 results in strong staining of myocytes and endothelial cells. MA1-620 cannot be used for immunoprecipitation.

In immunohistochemical studies, staining with MA1-620 is blocked by pre-incubating the sample with aldosterone. MA1-620 can also be used in formalin-fixed, paraffin-embedded sections. Due to the extremely low levels of MR expressed in native tissues, it is recommended that enhanced detection systems be used for Western blotting such as enhanced chemiluminescence. MA1-620 blocks the binding of aldosterone since it is directed against the steroid binding domain of the mineralocorticoid receptor. The antibody can also react with the steroid binding domain for aldosterone on other receptors.

The MA1-620 immunogen is aldosterone-3. This antibody was produced using the anti-idiotypic method.

Target Information

Steroid receptors are ligand-dependent, intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate hormone. Mineralocorticoids are a family of steroids, secreted by the adrenal cortex, necessary for the regulation of a number of metabolic processes including electrolyte regulation. These compounds exert their effect through their interaction with the mineralocorticoid receptor (MR) and that complex's subsequent association with DNA. Given the function of mineralocorticoids, it is not surprising to find that the kidney is a primary target organ for mineralocorticoids and that this organ has been shown to contain MR. The corresponding gene for the mineralocorticoid receptor is NR3C2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Loss of central mineralocorticoid or glucocorticoid receptors impacts auditory nerve processing in the cochlea.

iScience

Marchetta P,Eckert P,Lukowski R,Ruth P,Singer W,Rüttiger L,Knipper M

MA1-620 was used in Immunohistochemistry to imply that stress hormone-dependent functions of central MR/GR contribute to "precognitive" sound processing in the cochlea.

Fri Mar 18 00:00:00 EDT 2022

Long-Term Programming of CD8 T Cell Immunity by Perinatal Exposure to Glucocorticoids.

Cell

Hong JY,Lim J,Carvalho F,Cho JY,Vaidyanathan B,Yu S,Annicelli C,Ip WKE,Medzhitov R

MA1-620 was used in Immunohistochemistry to demonstrate that perinatal stress can have long-term consequences on CD8 T cell immunity by altering HPA axis activity.

Thu Mar 05 00:00:00 EST 2020

Mineralocorticoid receptor is involved in the aldosterone pathway in human red blood cells.

American journal of translational research

Bordin L,Saccardi C,Donà G,Sabbadin C,Andrisani A,Ambrosini G,Plebani M,Brunati AM,Ragazzi E,Gizzo S,Armanini D

Published figure using NR3C2 monoclonal antibody (Product # MA1-620) in Western Blot

Mon May 09 00:00:00 EDT 2016

Mineralocorticoid receptor and heat shock protein expression levels in peripheral lymphocytes from war trauma-exposed men with and without PTSD.

Psychiatry research

Matić G,Vojnović Milutinović D,Nestorov J,Elaković I,Manitašević Jovanović S,Elzaedi YM,Perišić T,Dunđerski J,Damjanović S,Knežević G,Špirić Ž,Vermetten E,Savić D

MA1-620 was used in western blot to study whether there is a relationship between peripheral leukocyte mineralocorticoid and heat shock protein expression levels and exposure to war trauma

Fri Feb 28 00:00:00 EST 2014

Hypothalamic-pituitary-adrenocortical axis hypersensitivity and glucocorticoid receptor expression and function in women with polycystic ovary syndrome.

Experimental and clinical endocrinology & diabetes : official journal, German Society of Endocrinology [and] German Diabetes Association

Milutinović DV,Macut D,Božić I,Nestorov J,Damjanović S,Matić G

MA1-620 was used in western blot to investigate the molecular mechanisms responsible for the pathogenesis of polycystic ovary syndrome

Tue Nov 01 00:00:00 EDT 2011

Mineralocorticoid receptor expression in human venous smooth muscle cells: a potential role for aldosterone signaling in vein graft arterialization.

American journal of physiology. Heart and circulatory physiology

Bafford R,Sui XX,Park M,Miyahara T,Newfell BG,Jaffe IZ,Romero JR,Adler GK,Williams GH,Khalil RA,Conte MS

MA1-620 was used in immunohistochemistry to study the expression of mineralocorticoid receptor in human venous smooth muscle cells

Fri Jul 01 00:00:00 EDT 2011

Localization of mineralocorticoid receptors at mammalian synapses.

PloS one

Prager EM,Brielmaier J,Bergstrom HC,McGuire J,Johnson LR

MA1-620 was used in immunohistochemistry and western blot to show the presence of mineralocorticoid receptors in the amygdala synapses

Wed Dec 15 00:00:00 EST 2010

Localization of mineralocorticoid receptors at mammalian synapses.

PloS one

Prager EM,Brielmaier J,Bergstrom HC,McGuire J,Johnson LR

MA1-620 was used in immunohistochemistry and western blot to show the presence of mineralocorticoid receptors in the amygdala synapses

Wed Dec 15 00:00:00 EST 2010

Aldosterone and mineralocorticoid receptor antagonists modulate elastin and collagen deposition in human skin.

The Journal of investigative dermatology

Mitts TF,Bunda S,Wang Y,Hinek A

MA1-620 was used in western blot to study the mechanisms of aldosterone-stimulated extracellular matrix deposition in human skin.

Fri Oct 01 00:00:00 EDT 2010

Cranial irradiation modulates hypothalamic-pituitary-adrenal axis activity and corticosteroid receptor expression in the hippocampus of juvenile rat.

General physiology and biophysics

Velickovic N,Djordjevic A,Drakulic D,Stanojevic I,Secerov B,Horvat A

MA1-620 was used in western blot to investigate the effect of cranial irradiation on activation of HPA axis and corticosteroid receptor expression in juvenile rat hippocampus

Thu Apr 08 00:00:00 EDT 2010

Synergistic induction of osteopontin by aldosterone and inflammatory cytokines in mesangial cells.

Journal of cellular biochemistry

Gauer S,Hauser IA,Obermüller N,Holzmann Y,Geiger H,Goppelt-Struebe M

MA1-620 was used in western blot to study the synergistic induction of osteopontin by aldosterone and inflammatory cytokines in mesangial cells

Fri Feb 01 00:00:00 EST 2008

Stimulation of testosterone production in rat Leydig cells by aldosterone is mineralocorticoid receptor mediated.

Molecular and cellular endocrinology

Ge RS,Dong Q,Sottas CM,Latif SA,Morris DJ,Hardy MP

MA1-620 was used in immunohistochemistry to investigate the role of mineralocorticoid receptor on testosterone production in rat Leydig cells

Thu Nov 24 00:00:00 EST 2005

Mineralocorticoid receptor expression in late-gestation ovine fetal lung.

Journal of the Society for Gynecologic Investigation

Keller-Wood M,Wood CE,Hua Y,Zhang D

MA1-620 was used in immunohistochemistry to investigate the distribution of mineralocorticoid receptor in the lung of late-gestation sheep fetus

Tue Feb 01 00:00:00 EST 2005

The mineralocorticoid receptor may compensate for the loss of the glucocorticoid receptor at specific stages of mammary gland development.

Molecular endocrinology (Baltimore, Md.)

Kingsley-Kallesen M,Mukhopadhyay SS,Wyszomierski SL,Schanler S,Schütz G,Rosen JM

MA1-620 was used in immunohistochemistry to investigate the effect of mineralocorticoid receptor on glucocorticoid receptor at specific stages of mammary gland development.

Sun Sep 01 00:00:00 EDT 2002

The p23 molecular chaperones act at a late step in intracellular receptor action to differentially affect ligand efficacies.

Genes & development

Freeman BC,Felts SJ,Toft DO,Yamamoto KR

Published figure using NR3C2 monoclonal antibody (Product # MA1-620) in Immunoprecipitation

Tue Feb 15 00:00:00 EST 2000

Prerequisite for cardiac aldosterone action. Mineralocorticoid receptor and 11 beta-hydroxysteroid dehydrogenase in the human heart.

Circulation

Lombès M,Alfaidy N,Eugene E,Lessana A,Farman N,Bonvalet JP

MA1-620 was used in immunohistochemistry to check MR's expression in human heart at the mRNA and protein level as well as 11-HSD's activity and dependence on its cofactor

Sat Jul 15 00:00:00 EDT 1995

Human skin as target for aldosterone: coexpression of mineralocorticoid receptors and 11 beta-hydroxysteroid dehydrogenase.

The Journal of clinical endocrinology and metabolism

Kenouch S,Lombes M,Delahaye F,Eugene E,Bonvalet JP,Farman N

MA1-620 was used in immunohistochemistry to investigate the expression of mineralocorticoid receptor and 11 beta-hydroxysteroid dehydrogenase in epidermis and appendages

Tue Nov 01 00:00:00 EST 1994

Aldosterone antagonists destabilize the mineralocorticosteroid receptor.

The Biochemical journal

Couette B,Lombes M,Baulieu EE,Rafestin-Oblin ME

MA1-620 was used in blocking/activating experiment to investigate the changes of the mineralocorticosteroid receptor stability with aldosterone stimulation

Sun Mar 15 00:00:00 EST 1992

Immunohistochemical localization of renal mineralocorticoid receptor by using an anti-idiotypic antibody that is an internal image of aldosterone.

Proceedings of the National Academy of Sciences of the United States of America

Lombès M,Farman N,Oblin ME,Baulieu EE,Bonvalet JP,Erlanger BF,Gasc JM

MA1-620 was used in immunohistochemistry to investigate the distribution of mineralocorticoid receptor in rabbit kidney

Thu Feb 01 00:00:00 EST 1990

Bioinformatics

Protein Aliases: aldosterone receptor; Mineralocorticoid receptor; Mineralocorticoid receptor (aldosterone receptor); mineralocorticoid receptor 1; mineralocorticoid receptor 2; mineralocorticoid receptor delta; MR; nuclear hormone receptor; Nuclear receptor subfamily 3 group C member 2; nuclear receptor subfamily 3, group C, member 2 variant 3

Gene Aliases: MCR; MLR; MR; NR3C2; NR3C2VIT

UniProt ID: (Human) P08235, (Rat) P22199

Entrez Gene ID: (Chicken) 374131, (Human) 4306, (Rabbit) 100358449, (Rat) 25672

Function(s)

regulation of transcription from RNA polymerase II promoter intracellular steroid hormone receptor signaling pathway transcription, DNA-templated regulation of transcription, DNA-templated cellular sodium ion homeostasis excretion mineralocorticoid receptor signaling pathway regulation of cell proliferation steroid hormone mediated signaling pathway transcription initiation from RNA polymerase II promoter signal transduction

Process(es)

RNA polymerase II core promoter proximal region sequence-specific DNA binding DNA binding RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding steroid binding transcription factor activity, sequence-specific DNA binding steroid hormone receptor activity protein binding zinc ion binding sequence-specific DNA binding double-stranded DNA binding mineralocorticoid receptor activity hormone binding protein homodimerization activity protein heterodimerization activity

NR3C2 Monoclonal Antibody (H10E4C9F)

Product Details

MA1-620

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Neutralization (Neu)

Inhibition Assays (IA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

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