Immunofluorescent analysis of Mineralocorticoid Receptor using Mineralocorticoid Receptor Monoclonal antibody (H10E4C9F) (Product# MA1-620) shows staining in HEK293 cells. Mineralocorticoid Receptor staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Mineralocorticoid Receptor (Product# MA1-620) at a dilution of 1:20-1:200 over night at 4 °C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.
|Tested species reactivity||Chicken, Human, Rabbit, Rat|
|Published species reactivity||Rabbit, Rat, Sheep, Human, Mouse, Chicken|
|Host / Isotype||Mouse / IgG1|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1/20|
|Inhibition Assays (IA)||Assay Dependent|
|Immunocytochemistry (ICC)||Assay dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1/100|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-620 detects mineralocorticoid receptor (MR) in human, rat, rabbit and chicken tissues.
MA1-620 has been successfully used in Western blot, immunocytochemistry and immunohistochemistry procedures. By Western blot, this antibody detects a 116 kDa protein representing MR in rat heart homogenate. Immunohistochemical staining of MR in rabbit atrium with MA1-620 results in strong staining of myocytes and endothelial cells. MA1-620 cannot be used for immunoprecipitation.
In immunohistochemical studies, staining with MA1-620 is blocked by pre-incubating the sample with aldosterone. MA1-620 can also be used in formalin-fixed, paraffin-embedded sections. Due to the extremely low levels of MR expressed in native tissues, it is recommended that enhanced detection systems be used for Western blotting such as enhanced chemiluminescence. MA1-620 blocks the binding of aldosterone since it is directed against the steroid binding domain of the mineralocorticoid receptor. The antibody can also react with the steroid binding domain for aldosterone on other receptors.
The MA1-620 immunogen is aldosterone-3. This antibody was produced using the anti-idiotypic method.
Steroid receptors are ligand-dependent, intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate hormone. Mineralocorticoids are a family of steroids, secreted by the adrenal cortex, necessary for the regulation of a number of metabolic processes including electrolyte regulation. These compounds exert their effect through their interaction with the mineralocorticoid receptor (MR) and that complex's subsequent association with DNA. Given the function of mineralocorticoids, it is not surprising to find that the kidney is a primary target organ for mineralocorticoids and that this organ has been shown to contain MR.
The corresponding gene for the mineralocorticoid receptor is NR3C2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Mineralocorticoid receptor and heat shock protein expression levels in peripheral lymphocytes from war trauma-exposed men with and without PTSD.
MA1-620 was used in western blot to study whether there is a relationship between peripheral leukocyte mineralocorticoid and heat shock protein expression levels and exposure to war trauma
|Mati¿ G,Vojnovi¿ Milutinovi¿ D,Nestorov J,Elakovi¿ I,Manita¿evi¿ Jovanovi¿ S,Elzaedi YM,Peri¿i¿ T,Dun¿erski J,Damjanovi¿ S,Kne¿evi¿ G,¿piri¿ ¿,Vermetten E,Savi¿ D||Psychiatry research (215:379)||2014|
Hypothalamic-pituitary-adrenocortical axis hypersensitivity and glucocorticoid receptor expression and function in women with polycystic ovary syndrome.
MA1-620 was used in western blot to investigate the molecular mechanisms responsible for the pathogenesis of polycystic ovary syndrome
|Milutinovi¿ DV,Macut D,Bo¿i¿ I,Nestorov J,Damjanovi¿ S,Mati¿ G||Experimental and clinical endocrinology and diabetes : official journal, German Society of Endocrinology [and] German Diabetes Association (119:636)||2011|
Aldosterone and mineralocorticoid receptor antagonists modulate elastin and collagen deposition in human skin.
MA1-620 was used in western blot to study the mechanisms of aldosterone-stimulated extracellular matrix deposition in human skin.
|Mitts TF,Bunda S,Wang Y,Hinek A||The Journal of investigative dermatology (130:2396)||2010|
Cranial irradiation modulates hypothalamic-pituitary-adrenal axis activity and corticosteroid receptor expression in the hippocampus of juvenile rat.
MA1-620 was used in western blot to investigate the effect of cranial irradiation on activation of HPA axis and corticosteroid receptor expression in juvenile rat hippocampus
|Velickovic N,Djordjevic A,Drakulic D,Stanojevic I,Secerov B,Horvat A||General physiology and biophysics (28 Spec No:219)||2009|
Synergistic induction of osteopontin by aldosterone and inflammatory cytokines in mesangial cells.
MA1-620 was used in western blot to study the synergistic induction of osteopontin by aldosterone and inflammatory cytokines in mesangial cells
|Gauer S,Hauser IA,Obermüller N,Holzmann Y,Geiger H,Goppelt-Struebe M||Journal of cellular biochemistry (103:615)||2008|
Mineralocorticoid receptor expression in human venous smooth muscle cells: a potential role for aldosterone signaling in vein graft arterialization.
MA1-620 was used in immunohistochemistry to study the expression of mineralocorticoid receptor in human venous smooth muscle cells
|Bafford R,Sui XX,Park M,Miyahara T,Newfell BG,Jaffe IZ,Romero JR,Adler GK,Williams GH,Khalil RA,Conte MS||American journal of physiology. Heart and circulatory physiology (301:H41)||2011|
Stimulation of testosterone production in rat Leydig cells by aldosterone is mineralocorticoid receptor mediated.
MA1-620 was used in immunohistochemistry to investigate the role of mineralocorticoid receptor on testosterone production in rat Leydig cells
|Ge RS,Dong Q,Sottas CM,Latif SA,Morris DJ,Hardy MP||Molecular and cellular endocrinology (243:35)||2005|
Mineralocorticoid receptor expression in late-gestation ovine fetal lung.
MA1-620 was used in immunohistochemistry to investigate the distribution of mineralocorticoid receptor in the lung of late-gestation sheep fetus
|Keller-Wood M,Wood CE,Hua Y,Zhang D||Journal of the Society for Gynecologic Investigation (12:84)||2005|
The mineralocorticoid receptor may compensate for the loss of the glucocorticoid receptor at specific stages of mammary gland development.
MA1-620 was used in immunohistochemistry to investigate the effect of mineralocorticoid receptor on glucocorticoid receptor at specific stages of mammary gland development.
|Kingsley-Kallesen M,Mukhopadhyay SS,Wyszomierski SL,Schanler S,Schütz G,Rosen JM||Molecular endocrinology (Baltimore, Md.) (16:2008)||2002|
Prerequisite for cardiac aldosterone action. Mineralocorticoid receptor and 11 beta-hydroxysteroid dehydrogenase in the human heart.
MA1-620 was used in immunohistochemistry to check MR's expression in human heart at the mRNA and protein level as well as 11-HSD's activity and dependence on its cofactor
|Lombès M,Alfaidy N,Eugene E,Lessana A,Farman N,Bonvalet JP||Circulation (92:175)||1995|
Human skin as target for aldosterone: coexpression of mineralocorticoid receptors and 11 beta-hydroxysteroid dehydrogenase.
MA1-620 was used in immunohistochemistry to investigate the expression of mineralocorticoid receptor and 11 beta-hydroxysteroid dehydrogenase in epidermis and appendages
|Kenouch S,Lombes M,Delahaye F,Eugene E,Bonvalet JP,Farman N||The Journal of clinical endocrinology and metabolism (79:1334)||1994|
Immunohistochemical localization of renal mineralocorticoid receptor by using an anti-idiotypic antibody that is an internal image of aldosterone.
MA1-620 was used in immunohistochemistry to investigate the distribution of mineralocorticoid receptor in rabbit kidney
|Lombès M,Farman N,Oblin ME,Baulieu EE,Bonvalet JP,Erlanger BF,Gasc JM||Proceedings of the National Academy of Sciences of the United States of America (87:1086)||1990|
The p23 molecular chaperones act at a late step in intracellular receptor action to differentially affect ligand efficacies.
MA1-620 was used in immunoprecipitation to investigate the role of p23 molecular chaperones in intracellular receptor function in animal and yeast cells.
|Freeman BC,Felts SJ,Toft DO,Yamamoto KR||Genes and development (14:422)||2000|
Aldosterone antagonists destabilize the mineralocorticosteroid receptor.
MA1-620 was used in blocking/activating experiment to investigate the changes of the mineralocorticosteroid receptor stability with aldosterone stimulation
|Couette B,Lombes M,Baulieu EE,Rafestin-Oblin ME||The Biochemical journal (282 ( Pt 3):697)||1992|