|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||BALB/c mouse immunized with Baculovirus-expressed recombinant human SF-1 (220-461aa).|
|Purification||Ammonium sulfate precipitation|
|Storage buffer||physiological saline|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||0.1 µg/ml|
|Immunohistochemistry (IHC)||10 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||10 µg/ml|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||3 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Steroidogenic factor-1 (SF-1) is an orphan nuclear receptor involved in organ development and differentiation of the endocrine system. SF-1 has been shown to be essential for fetal development of the hypothalamus, pituitary gland, gonads, and the adrenal gland. SF-1 has been shown to promote transcriptional activity of many steroidogenic enzymes from the cytochrome P450 family. SF-1 contains two activation function domains, designated AF1 and AF2, common to many nuclear steroid receptors which have been shown to be necessary for interaction with other nuclear receptors as well as coactivators and corepressors including steroid receptor coactivator-1 (SRC-1), glucocorticoid receptor interacting protein 1 (GRIP1), cAMP response element binding protein (CREB) binding protein (CBP) and silencing mediator of retinoid acid and thyroid hormone receptor (SMRT).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Steroidogenic factor 1 promotes aggressive growth of castration-resistant prostate cancer cells by stimulating steroid synthesis and cell proliferation.
434200 was used in immunohistochemistry - paraffin section to test if steroidogenic factor is expressed in castration-resistant prostate cancer and determine if it stimulates aberrant steroidogenesis and fuels aggressive growth.
|Lewis SR,Hedman CJ,Ziegler T,Ricke WA,Jorgensen JS||Endocrinology (155:358)||2014|
Ovarian Brenner tumour: a morphologic and immunohistochemical analysis suggesting an origin from fallopian tube epithelium.
434200 was used in immunohistochemistry to identify the origin of ovarian Brenner tumour
|Kuhn E,Ayhan A,Shih IeM,Seidman JD,Kurman RJ||European journal of cancer (Oxford, England : 1990) (49:3839)||2013|
Elevated levels of the steroidogenic factor 1 are associated with over-expression of CYP19 in an oestrogen-producing testicular Leydig cell tumour.
434200 was used in immunohistochemistry (paraffin) to study CYP19 over-expression in testicular Leydig cell tumors.
|Straume AH,Løvås K,Miletic H,Gravdal K,Lønning PE,Knappskog S||European journal of endocrinology (166:941)||2012|
ChIP-on-chip analysis reveals angiopoietin 2 (Ang2, ANGPT2) as a novel target of steroidogenic factor-1 (SF-1, NR5A1) in the human adrenal gland.
434200 was used in immunohistochemistry - frozen section to identify Ang2 as a novel target of the nuclear receptor steroidogenic factor-1 in the adrenal gland.
|Ferraz-de-Souza B,Lin L,Shah S,Jina N,Hubank M,Dattani MT,Achermann JC||FASEB journal : official publication of the Federation of American Societies for Experimental Biology (25:1166)||2011|