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| Applications | Tested Dilution | Publications |
|---|---|---|
Western Blot (WB) |
1:500-1:2,000 | - |
Flow Cytometry (Flow) |
1:100 | - |
| Product Specifications | |
|---|---|
Species Reactivity |
Human |
Host/Isotype |
Mouse / IgG1 |
Class |
Monoclonal |
Type |
Antibody |
Clone |
OTI5F8 |
Immunogen |
Human recombinant protein fragment corresponding to amino acids 1-250 of human NUBPL produced in E.coli. |
Conjugate |
Unconjugated |
Form |
lyophilized |
Concentration |
1 mg/mL |
Purification |
Affinity chromatography |
Storage buffer |
PBS, pH 7.3, with 8% trehalose |
Contains |
no preservative |
Storage conditions |
-20° C, Avoid Freeze/Thaw Cycles |
For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
Required for the assembly of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I). May deliver of one or more Fe-S clusters to complex I subunits.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: huInd1; IND1 homolog; Iron-sulfur protein NUBPL; iron-sulfur protein required for NADH dehydrogenase; nucleotide binding protein-like; Nucleotide-binding protein-like
Gene Aliases: C14orf127; huInd1; IND1; NUBPL
UniProt ID: (Human) Q8TB37
Entrez Gene ID: (Human) 80224
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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