Invitrogen

NUP107 Monoclonal Antibody (39C7)

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NUP107 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of NUP107 was performed using 70% confluent log phase PC-3 cells. The cells were fixed with 4% Paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 10 minutes at room temperature. The cells were labeled with NUP107 Monoclonal Antibody (39C7) (Product # MA1-10031) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32723, 1:2000 dilution) for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear membrane localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

NUP107 Antibody in Western Blot (WB) — Western blot analysis of NUP107 in different cell lysates using an NUP107 monoclonal antibody (Product # MA1-10031) at a dilution of 1:100 seen in green. 1) protein standard (red), 2) HEK293 cytosol, 3) HEK293 nuclear, 4) NIH-3T3 cytosol, 5) NIH-3T3 nuclear, 6) HeLa cytosol, and 7) HeLa nuclear fraction lysate. The band observed at 68 kDa represents the target NUP107 protein.

Product Details

MA1-10031

Applications	Tested Dilution	Publications
Western Blot (WB)	1:100	-
Immunocytochemistry (ICC/IF)	1:100-1:500	View 2 publications 2 publications

Product Specifications
Species Reactivity	Bovine, Chicken, Fruit fly, Horse, Human, Mouse, Pig, Rat, Yeast
Published species	Human
Host/Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Clone	39C7
Immunogen	Yeast nuclear preparation.
Conjugate	Unconjugated
Form	Liquid
Concentration	Conc. Not Determined
Storage buffer	tissue culture supernatant
Contains	5mM sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2154446

Product Specific Information

For immunofluorescence on yeast cells, try MCA-39C7 diluted 1:100 to 1:500. For immunofluorescence on mammalian cells try at 1:50 to 1:100.

Target Information

The nuclear pore complex (NPC) is a protein assembly localized at the nuclear rim and mediates macromolecular transport between the nucleus and the cytoplasm. The mammalian nucleoporin (NUP)-107 is part of the hetero-oligomeric complex that also contains NUP160, NUP133, NUP96, and mammalian homolog of yeast sec13p. While the majority of the NUP107-160 nuclear pore sub-complex localizes to the nuclear pore, a small fraction is observed at kinetochores and pro-metaphase spindle poles in mitotic cells in association with proteins such as Mad1, Mad2, Bub3 and Cdc20. Immunodepletion of the NUP107-160 complex resulted in defective spindle assembly indicating that it has multiple functions. NUP107 has recently been identified as an HIV dependency factor (HDF), suggesting that NUP107 may be an important drug target in HIV treatment. Multiple isoforms of NUP107 are known to exist.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Nuclear Pore Complexes Cluster in Dysmorphic Nuclei of Normal and Progeria Cells during Replicative Senescence.

Cells

Röhrl JM,Arnold R,Djabali K

Published figure using NUP107 monoclonal antibody (Product # MA1-10031) in Immunocytochemistry

Thu Jan 14 00:00:00 EST 2021

G₄C₂ Repeat RNA Initiates a POM121-Mediated Reduction in Specific Nucleoporins in C9orf72 ALS/FTD.

Neuron

Coyne AN,Zaepfel BL,Hayes L,Fitchman B,Salzberg Y,Luo EC,Bowen K,Trost H,Aigner S,Rigo F,Yeo GW,Harel A,Svendsen CN,Sareen D,Rothstein JD

MA1-10031 was used in Immunocytochemistry to suggest that the expression of expanded C9orf72 ALS/FTD repeat RNA alone affects nuclear POM121 expression in the initiation of a pathological cascade affecting nucleoporin levels within neuronal nuclei and ultimately downstream neuronal survival.

Wed Sep 23 00:00:00 EDT 2020

Bioinformatics

Protein Aliases: 107 kDa nucleoporin; CG6743-PA; nuclear pore complex; Nuclear pore complex protein Nup107; Nuclear pore complex protein Nup107-like protein; Nuclear pore protein nup107; nucleoporin 107; nucleoporin 107kDa; Nucleoporin Nup107; Nup107-PA; Nup170; p105

Gene Aliases: AW541137; BcDNA:LD18761; C76801; CG6743; Dmel\CG6743; Dmel_CG6743; dmNup107; NPHS11; Nup; Nup 107; NUP107; Nup170; NUP84; SPBC428.01c; SPBC582.11c

UniProt ID: (Human) P57740, (Fruit fly) Q9V466, (Mouse) Q8BH74, (Fission yeast) Q10331

Entrez Gene ID: (Human) 57122, (Horse) 100059287, (Chicken) 101751988, (Pig) 100516909, (Fruit fly) 34481, (Rat) 116555, (Mouse) 103468, (Fission yeast) 3361255

Molecular Function: transporter