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Na+/H+ Exchanger 1 (NHE-1) (extracellular) Polyclonal Antibody

View all (17) Nhe-1 antibodies

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Datasheet

Protocols

Questions & Answers

Tech Support

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Na+/H+ Exchanger 1 (NHE-1) (extracellular) Antibody in Immunocytochemistry (ICC/IF) — Expression of NHE1 in human MCF-7 cells - Cell surface detection of NHE1 in live intact human MCF-7 breast adenocarcinoma cells. A. Extracellular labeling of cells with Anti-Na+/H+Exchanger 1 (NHE-1) (extracellular) Antibody (#ANX-010), (1:25), followed by goat Anti-rabbit-AlexaFluor-594 secondary Antibody (red). B. Live view of the cells. C. Merge of the two pictures.

Na+/H+ Exchanger 1 (NHE-1) (extracellular) Antibody in Immunohistochemistry (Frozen) (IHC (F)) — Expression of NHE1 in mouse substantia nigra pars compacta - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-Na+/H+Exchanger 1 (NHE-1) (extracellular) Antibody (#ANX-010), (1:200), followed by goat- Anti-rabbit-AlexaFluor-488. NHE1 immunoreactivity (green) appears in neuronal profiles (arrows). Cell nuclei are stained with DAPI (blue).

Na+/H+ Exchanger 1 (NHE-1) (extracellular) Antibody in Western Blot (WB) — Western blot analysis of rat brain membranes (lanes 1 and 6), mouse brain lysate (lanes 2 and 7), human MCF-7 breast adenocarcinoma cells (lanes 3 and 8), human U-87 MG glioblastoma cells (lanes 4 and 9) and human THP-1 acute monocytic leukemia cells (lanes 5 and 10): - 1-5. Anti-Na+/H+Exchanger 1 (NHE-1) (extracellular) Antibody (#ANX-010), (1:200).6-10. Anti-Na+/H+Exchanger 1 (NHE-1) (extracellular) Antibody , preincubated with Na+/H+ Exchanger 1/NHE-1 (extracellular) Blocking Peptide (#BLP-NX010).

Na+/H+ Exchanger 1 (NHE-1) (extracellular) Antibody in Flow Cytometry (Flow) — Cell surface detection of NHE1 by indirect flow cytometry in live intact human MEG-01 megakaryocytic leukemia cells: - (black line) cells. (red) Cells + goat- Anti-rabbit-FITC. (green) Cells + Anti-Na+/H+ Exchanger 1 (NHE-1) (extracellular) Antibody (#ANX-010), (2.5μg) + goat- Anti-rabbit-FITC.

Product Details

ANX-010-200UL

Applications	Tested Dilution	Publications
Western Blot (WB)	1:200	-
Immunohistochemistry (Frozen) (IHC (F))	1:200	-
Immunocytochemistry (ICC/IF)	1:25	-
Flow Cytometry (Flow)	2.5 µg	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	(C)RERSIGDVTTAPSE, corresponding to amino acid residues 54-67 of rat NHE-1, 1st extracellular loop View immunogen
Conjugate	Unconjugated
Form	Lyophilized
Concentration	0.8 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.4, with 1% BSA
Contains	0.05% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles

Product Specific Information

Reconstitution: 1 X 25 µL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Information

The Na+/H+ antiporter (Nhe-1) is a ubiquitous membrane-bound enzyme involved in pH regulation of vertebrate cells and is specifically inhibited by the diuretic drug amiloride and activated by a variety of signals including growth factors, mitogens, neurotransmitters, and tumor promoters. Nhe-1 acts as an anchor for actin filaments to control the integrity of the cortical cytoskeleton. This occurs through a previously unrecognized structural link between Nhe-1 and the actin-binding proteins ezrin, radixin, and moesin, collectively referred to as ERM proteins. A structural role for Nhe-1 has been proposed in regulating the cortical cytoskeleton that is independent of its function as an ion exchanger. It is also thought that Nhe-1 play a role in hypertension. At least two isoforms of Nhe-1 are known to exist.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: APNH; Na(+)/H(+) antiporter, amiloride-sensitive; Na(+)/H(+) exchanger 1; Na+/H+ antiporter; Na+/H+, amiloride sensitive; Na-Li countertransporter; NHE-1; protein phosphatase 1, regulatory subunit 143; slow-wave epilepsy; Sodium/hydrogen exchanger 1; Solute carrier family 9 (sodium/hydrogen exchanger 1), antiporter, Na+/H+, (amiloride sensitive); solute carrier family 9 (sodium/hydrogen exchanger), isoform 1 (antiporter, Na+/H+, amiloride sensitive); solute carrier family 9 (sodium/hydrogen exchanger), member 1; solute carrier family 9 (sodium/hydrogen exchanger), member 1 (antiporter, Na+/H+, amiloride sensitive); Solute carrier family 9 member 1; solute carrier family 9, member 1; solute carrier family 9, subfamily A (NHE1, cation proton antiporter 1), member 1

Gene Aliases: APNH; APNH1; AW554487; LIKNS; mir-5122; Mir5122; NHE-1; NHE1; PPP1R143; SLC9A1; swe

UniProt ID: (Human) P19634, (Rat) P26431, (Mouse) Q61165

Entrez Gene ID: (Human) 6548, (Rat) 24782, (Mouse) 20544

Molecular Function: transporter secondary carrier transporter