|Tested species reactivity||Mouse, Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to amino acids 251-266 of human Nur77 (Nak-1).|
|Storage buffer||PBS with 0.05% BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|ChIP assay (ChIP)||1:10-1:500|
|Western Blot (WB)||1:500-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Suggested positive control: MOLT-4 or NIH-3T3.
Nur77 (Nak-1) is a member of the steroid/thyroid hormone receptor superfamily. The gene for Nur77 is induced rapidly by androgens/growth factors and may have functions related to cell proliferation, differentiation and apoptosis (1). Liu et al (2) have demonstrated that mouse nur77 is necessary for induced apoptosis in T-cell hybridomas and can also be induced during early mitogenesis. Nur77 (Nak-1) is a phosphoprotein and its size ranges from 67 kD to 88 kD, depending on post-translational modifications.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Nucleoside, nucleotide and nucleic acid metabolism mRNA transcription mRNA transcription regulation Signal transduction Immunity and defense Stress response Developmental processes Ectoderm development Neurogenesis