Immunofluorescent analysis of Nanog (green) showing nuclear staining of NCCIT cells (right panel) compared to negative HeLa cell control (left panel). The cells were fixed with formalin for 15 minutes, permeabilized with 0.1% Triton X-100 in TBS, washed, and then blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a DyLight 488-conjugated Nanog monoclonal antibody (Product # MA1-017-D488) in 3% BSA-PBS at a dilution of 1:20 and incubated for 1 hour at 37C in the dark. F-Actin (left panel, red) was stained with DyLight 554 Phalloidin (Product # 21834) and nuclei (left panel, blue) were stained with DAPI. Images were taken at 60X magnification.
|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG|
|Immunogen||Full-length human recombinant protein expressed in bacteria|
|Storage buffer||PBS with proprietary stabilizer|
|Contains||0.02% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-017-D488 has been successfully used in ICC/IF applications with human samples.
MA1-017-D488 can be used for flow cytometry analysis of Nanog in human iPSCs.
Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic lineages.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.